Deepthi Chandran, B (2022) Evaluation of Human Fetal Osteoblast Proliferation and Morphology on Titanium Infrazygomatic Mini-Implants: An In Vitro Study. Masters thesis, Ragas Dental College and Hospital, Chennai.
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Abstract
AIM OF THE STUDY: The aim of this in vitro study was to: 1. Evaluating the proliferation and morphology of human fetal osteoblast (hFOB) cells seeded on the surface of two different brands of titanium infrazygomatic (IZC) mini-implants (Bioray & Dentos) in three different time periods (24, 48 & 72 hours). 2. Assess the chemical composition on the surface of two different brands of titanium IZC mini-implants in three different region (head, body & tip) and detailing the chemical component profile. MATERIALS AND METHOD: Six experimental units of two brands of titanium IZC mini-implants Dentos, (korea) (n=6) and Bioray (n=6) were used as a test material and polystyrene disk (n=3) was used as a control. hFOB cells seeded on the surface of sterilized titanium IZC mini-implants and polystyrene disk in three different time period (24, 48 and 72 hours). hFOB cells adhesion, proliferation was assessed quantitatively and morphology was assessed qualitatively in three time periods. As-received one titanium IZC mini-implant from each brands were subjected to surface composition analysis (head, body and tip) using Energy Dispersive X-ray analysis. RESULTS: 1. Quantitative evaluation showed there was a significant increase in number of proliferated hFOB cells in both the titanium IZC mini-implants in three different time periods. One way ANOVA showed there was a statistical significant difference in the proliferated hFOB cells between the two titanium IZC mini-implants (p=0.000). Post hoc and Tukey tests also revealed there was a statistically significant difference between two titanium IZC mini-implants, Bioray IZC mini-implant had more number of proliferated hFOB cells in three different time period than Dentos IZC mini-implant (p=0.001, 0.028 & 0.000). Comparing both IZC mini-implants with polystyrene, polystyrene had more number of proliferated hFOB cells (p=0.000). Qualitative evaluation showed cell morphological change was observed on the surface (body, (thread & pitch) and tip) of titanium IZC mini-implants and surface of polystyrene disk in three different time periods (24, 48 & 72 hours). 2. The significant and most frequent element present on the surface of both the brands of titanium IZC mini-implants were titanium, aluminium and vanadium. Maximum amount of titanium was present on head portion of Bioray IZC mini-implant (90.10%) followed by body and tip (89.31%, 88.12%) compared with Dentos IZC mini-implant. CONCLUSION: Time influenced hFOB cell proliferation was observed on all the surfaces of titanium IZC mini-implant (Dentos and Bioray) with lowest cell count values was at 24 hours, intermediate at 48 hours and highest at 72 hours which was statistical significant. More proliferation of hFOB cells was observed on the surface of polystyrene disk compared to IZC mini-implants (Bioray & Dentos) due to its high affinity. Comparing the hFOB cell proliferation between the titanium IZC mini-implant, Bioray titanium IZC mini-implant showed maximum number of proliferated hFOB cells in all three time periods. The increased cell count is attributed to the increased elemental composition of titanium on all its surfaces (head, body and tip) of the Bioray IZC mini-implant.
| Item Type: | Thesis (Masters) |
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| Additional Information: | Reg.No.241919154 |
| Uncontrolled Keywords: | Human fetal osteoblast cell (hFOB), Titanium infrazygomatic mini-implants, cell culture, scanning electron microscopy (SEM), Energy Dispersive X-ray analysis (EDAX). |
| Subjects: | DENTAL > Orthodontics and Dentofacial Orthopedics |
| Depositing User: | Subramani R |
| Date Deposited: | 08 May 2022 10:58 |
| Last Modified: | 23 Mar 2024 06:22 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/19830 |
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