Saratha, R (2017) Pharmacological Evaluation of Ethanolic Extract of Withania Coagulans Dunal (Flower Buds). Masters thesis, Periyar College of Pharmaceutical Sciences, Tiruchirappalli.
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Abstract
OBJECTIVE OF THE WORK:The plants are the key source of medicine in Ayurveda for treatment and prevention of diseases and maintenance of healthy life. The plants are used in medicine since antiquity. Much of the medicinal plants are documented in the Ancient Ayurvedic classics and these plants are still used successfully to treat different ailments. One of these plants which is used to treat various disease is Withania coagulans Dunal. The shrub is important for the property of coagulating milk, possessed by its berries; they are used for this purpose in North-West India and adjoining country. Withania coagulans are the most reputed medicinal plants of Ayurveda and has well-descript pharmacological activities such as physiological and metabolic restoration, anti-arthritic, anti-aging, cognitive function, improvement in geriatric states and recovery from neurodegenerative disorders.75, 132 Based on the literature review, it was planned to carry out the biological screening as outlined below To validate acute oral toxicity To study the behavioral coordination in rats To study the antiobesity activity in progesterone induced obese rats To study the antihyperlipidemic activity in fructose induced hyperlipidemic rats To study the analgesic activity To evaluate the antimicrobial activity and anthelmintic activity. CONCLUSION: From the study entitled "Pharmacological Evaluation of Withania coagulans Dunal (Flower buds), the following conclusion could be drawn The present study has thus duly supported the traditional use of Ethanolic Extract of Withania coagulans Dunal have scientifically proved the behavioral coordination, antiobesity, antihyperlipidemic, analgesic, anthelmintic, and antimicrobial activity. Apart from the suggested actions listed in discussion part absence of acute toxicity may also offer a new hope for safe treatment in future. Preliminary phytochemical study ethanolic extract of Withania coagulans Dunal was found to contain Carbohydrates, Protein, Steroids, Flavanoids, Alkaloids, Glycosides and Amino Acids are present. Presence of Alkaloids, Flavanoids and Glycosides in the ethanolic extract of Withania coagulans Dunal was concluded by IR & HPTLC analysis. Though present in small quantities, it was found to produce considerable effects The results of the present study indicate that the ethanolic extract of Withania coagulans was non-toxic up to dose level of 2000mg/kg body weight in albino rats as per acute oral toxicity studies. 1/10th of the LD50 Dose is 200mg/kg is used for Pharmacological screening Evaluation of anti-obesity activity of ethanolic extract of Withania coagulans showed significant anti obesity property by the obtained significant results against Progesterone induced obesity. From the results observed in the Progesterone induced obesity, it may be concluded that the test compound at the dose of 200mg/kg body weight displays a significant anti- obesity activity compared to standard drug Orlistat. The ethanolic extract of Withania coagulans at a dose of 200mg/kg exhibited significant hypolipidemic activity in Fructose induced hyperlipidemic rats. This is showed by the reduction of serum lipid parameters such as triglycerides, total cholesterol, LDL, VLDL, SGOT and SGPT with an increase in HDL concentration in the group treated with 200mg/kg of Ethanolic Extract of Withania coagulans. It is found that there is a significant reduction in the Serum glucose. The ethanolic extract of Withania coagulans was subjected to the Tail Flick Analgesic test, showed a significant inhibitory effect. The ethanolic extract of Withania coagulans was subjected to the Anthelmintic activity against Pheritima posthuma, showed a significant inhibitory effect at higher doses 75mg/10ml of Normal Saline & 100mg/10ml of Normal Saline. The ethanolic extract of Withania coagulans was subjected to the antimicrobial test, showed a significant inhibitory effect on both gram positive, gram negative bacteria and also fungi. In future, further investigation might provide an insight to identify the functional groups in the ethanolic extract of Withania coagulans active responsible for the behavioral coordination, antiobesity, antihyperlipidemic, analgesic, anthelmintic, and antimicrobial effect and to elucidate the exact mechanism of action, which is responsible for the observed significant activity with low toxicity and better therapeutic index
| Item Type: | Thesis (Masters) |
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| Uncontrolled Keywords: | Pharmacological Evaluation ; Ethanolic Extract ; Withania Coagulans Dunal ; Flower Buds |
| Subjects: | PHARMACY > Pharmacology |
| Depositing User: | Ravindran C |
| Date Deposited: | 08 Jan 2018 08:17 |
| Last Modified: | 08 Jan 2018 08:17 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/5290 |
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