Sujith, K (2012) Neuropharmacological Profile of Indian Medicinal Plant. Doctoral thesis, The Tamilnadu Dr. M.G.R. Medical University, Chennai.
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Abstract
Medicinal herbs are indispensible part of traditional medicine practiced all over the world due to easy access, low cost, least risk and low side effect profile. Anacyclus pyrethrum, Family Asteraceae is used in traditional system of medicine and it is regarded as a tonic to the nervous system. The plant roots are reported for antibacterial, anti-inflammatory, immunostimulating and aphrodisiac activities. As root of this plant has potential health benefits, it can be better utilized for nutraceutical and functional food formulations. The present work was undertaken to study neuropharmacological role of Anacyclus pyrethrum in consideration to memory improvement activity to treat the diseases related to cognition and use in learning and memory processes. • In phytochemical analysis ethanolic extract of roots of Anacyclus pyrethrum showed more number of phytoconstituents. • Ethanolic extract of Anacyclus pyrethrum was found have significant inhibition on acetylcholinesterase activity by spectrophotometric and TLC plate bioassay detection for AChE inhibition. • TLC and HPTLC of ethanolic extract of Anacyclus pyrethrum were done. The good resolution of spots in TLC and HPTLC shows the presence of various active principles in the extract. • Total phenolic, flavanoid, ascorbic content in ethanolic extract of Anacyclus pyrethrum was quantified. • In the acute toxicity study, ethanolic extract of Anacyclus pyrethrum were found to be non-toxic and did not produce any adverse effects upto a dose level of 2000 mg/kg bodyweight. • In the chronic toxicity study for 90 days extract treated groups showed no changes in body weight, food and water intake as compared to control group. There was no treatment related adverse effect of Anacyclus pyrethrum extract on haematological, biochemical parameters and histopathological studies in rats. • General behaviour studies on ethanolic extract of Anacyclus pyrethrum has not shown any deviation in treated rats from normal behaviour and suggests that ethanolic extract does not possess any neurotoxicity. • The experimental observation using actophotometer shows that extracts of Anacyclus pyrethrum did not produce significant effect on the locomotor activity. • Experimental findings on ethanolic extract of Anacyclus pyrethrum suggest the extracts did not demonstrate any effect on the muscle coordination, as indicated by the findings with respect to the rotarod experiment. • Study of exploratory activity using hole board apparatus and elevated plus maze in rats reveals that ethanolic extract of Anacyclus pyrethrum treated rats does not possess anxiolytic activity. • The antidepressant evaluation models using forced swim test and tail suspension test demonstrated that ethanolic extract of Anacyclus pyrethrum clearly posses an antidepressant action in rats. • Effect of ethanolic extract of Anacyclus pyrethrum on scopolamine induced interoceptive behavior models revealed the antiamnesic potential of Anacyclus pyrethrum. • In the present study Anacyclus pyrethrum inhibited acetylcholinestrease enzyme, there by elevating acetylcholine concentration in the brain homogenate and ultimately improved memory in rats treated with scopolamine. • Decreased activities of enzymatic and non-enzymatic antioxidants together with increased levels of lipid peroxidation in rat brain seen in scopolamine induced interoceptive behavior models in rats and were brought back to normal levels by the administration of ethanolic extract of Anacyclus pyrethrum. • Rats treated with ethanolic extract of Anacyclus pyrethrum in extroceptive behaviour models for learning and memory using social test model demostrated that ethanolic extract of Anacyclus pyrethrum display short term social memory enhancing property. • Rats treated with ethanolic extract of Anacyclus pyrethrum in extroceptive behaviour models for learning and memory using elevated plus maze revealed significant improvement in memory thus demonstrating nootropic activity. • Repeated administration of the extracts showed significant changes in neurotransmitter level. There was decrease in dopamine level and increase in serotonin level when compared to control group and there were no effect on level of glutamate on repeated administration of extract. • Ethanolic extract of Anacyclus pyrethrum showed good antioxidant property, when studied by the in vitro experimental methods such as DPPH scavenging activity, hydroxyl radical scavenging activity, hydrogen peroxide scavenging activity, nitric oxide scavenging activity and also reducing power of the extract is associated with its antioxidant property. • Exvivo methods such as lipid peroxidation assay also suggest antioxidant potential of the extract. In conclusion, the present study revealed ethanolic extract of Anacyclus pyrethrum have memory enhancing property, antidepressant activity and neuroprotective role which may be due to its anticholinesterase potential, effect on neurotransmitters such as dopamine and serotonin level and its antioxidant profile, therefore it seem that Anacyclus pyrethrum may prove to be useful in treatment of dementia of Alzhemier diseases. Further studies are to be warranted for its safety, efficacy and mechanism of action for its therapeutic potential.
| Item Type: | Thesis (Doctoral) |
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| Uncontrolled Keywords: | Medicinal Plant, Neuropharmacological Profile, India. |
| Subjects: | PHARMACY > Pharmacy Practice |
| Depositing User: | Devi S |
| Date Deposited: | 28 Jun 2017 07:50 |
| Last Modified: | 11 Sep 2022 12:27 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/525 |
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