Saravanan, R (2017) Comparative Evaluation of Various Probiotic Strains on Inhibition of Streptococcus Mutans: An In Vitro study. Masters thesis, Tamil Nadu Government Dental College and Hospital, Chennai.
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Abstract
AIM AND OBJECTIVES : The aim of this in vitro study was to investigate the ability of selected lactobacilli strains used in commercially available probiotic products, to inhibit growth of oral mutans streptococci The objectives were to evaluate the inhibition of S. mutans (Standard MTCC and clinical strains) by probiotic cell free supernatant and live cells and to evaluate the inhibition of S. mutans by probiotic combinations. METHODOLOGY : Lawn cultures of S. mutans were made on MHA plates supplemented with 5% sheep blood agar. Sterile cotton swabs were dipped in the broth cultures of S. mutans and the inoculums were seeded in three different directions to form lawn cultures. Plates were allowed to dry for 10 minutes, wells of 8mm diameter were punched using sterile cork borer.25, 50, 100, 150μl of the 48hrs broth cultures and cell free supernatants of the probotic strains were added and the plates were incubated at 37°c at 5% CO2 .Diameter of zone of inhibition around the wells was measured in mm using HiAntibiotic zone scale after 24hrs of inhibition. Lawn cultures of S. mutans were made in MSA plates supplemented with 5% sheep blood agar. Sterile cotton swabs dipped in fresh broth cultures of S. mutans is inoculated and seeded to lawn culture and the plates were allowed to dry for 10 mins .Wells of 8mm diameter were punched using sterile cork borer and 200μl of 48 hrs broth cultures and cell free supernatant combinations of probiotic strains were added in the presence of 5% CO2 .Diameter of zone of inhibition was measured around the wells using HiAntibiotic zone scale in mm after incubation of 24 hrs. S. mutans were incorporated in the molten agar at 0.5% w/v and poured into sterile petriplates and allowed to solidify. Wells of 8mm diameter were punched using sterile cork borer 25, 50, 100, 150μl of broth cultures and cell free supernatant of the probiotic strains were added in the wells using sterile microtips. The diameter of zone of inhibition was measured in mm using HiAntibiotic zone scale after incubation of 48 hrs in the presence of 5% CO2 at 37°c . RESULTS : Results showed that L. fermentum and L. rhamnosus demonstrated the largest zones of clearance for both clinical and standard MTCC strains of Mutans streptococci. The weakest zone of inhibition of mutans streptococci was displayed by L acidophilus and L.amylovorus. Both cell free supernatant and live cells of probiotic strains of L. fermentum and L. rhamnosus could significantly inhibit clinical and standard MTCC strains of Mutans streptococci. The probiotics in higher concentrations such as in 100μl, 125μl, 150μl were able to significantly inhibit Mutans streptococci when compared to lower concentrations. The combinations of various probiotic strains better inhibited pathogenic mutans streptococci rather than individual strains. CONCLUSION : The present study concluded that live probiotics and/or cell-free probiotics are capable of inhibiting S. mutans. The probiotic strains in combinations better inhibited S. mutans than individual strains.
| Item Type: | Thesis (Masters) |
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| Uncontrolled Keywords: | Probiotics, S. mutans, zone of inhibition, culture. |
| Subjects: | DENTAL > Conservative Dentistry and Endodontics |
| Depositing User: | Kambaraman B |
| Date Deposited: | 04 Jan 2018 03:53 |
| Last Modified: | 04 Jan 2018 03:53 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/5187 |
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