Vaishnavi, Sivasankar (2013) Growth Characteristics and Expression of CD73 and CD146 in the Cells Cultured from Dental Pulp: A Flow Cytometry and Immunocytochemical study. Masters thesis, Ragas Dental College and Hospital, Chennai.
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Abstract
BACKGROUND: Dental Pulp Stem Cells (DPSCs) in permanent teeth and Stem Cells from Human Exfoliated Deciduous (SHED) teeth are a source of adult mesenchymal stem cells. Very little is known of the similarities and differences between DPSCs and SHED during their early passage in cultures. AIMS AND OBJECTIVES: To culture stem cells from the pulp of deciduous and permanent teeth and compare their growth characteristics, morphology and immuno-phenotype using mesenchymal stem cell markers: CD146 and CD73 in their 1st, 3rd and 5th passage of culture. MATERIALS AND METHODS: 15 teeth were obtained for isolation of dental pulp of which 12 were permanent teeth and 3 were deciduous teeth. Growth characteristics, morphology and colony forming efficiency were assessed for the deciduous and permanent samples. Immunocytochemistry and flow cytometry using CD146 and CD73 was performed in deciduous and permanent samples in the 1st, 3rd and 5th passage of culture. Data was analyzed using SPSS TM software (version 17.0.0). RESULTS: Seven of the fifteen teeth cultured yielded sufficient cells for characterization. The time taken to reach confluency and the population doubling time was lower in SHED compared to DPSCs, and the colony forming unit efficiency was higher in SHED compared to DPSCs but the results were not statistically significant. The seeding efficiency was significantly higher in SHED compared to DPSCs (P=0.046). There was a decrease in mitotic phenotype and increase in post-mitotic phenotype in both deciduous and permanent samples from day 1 to day 8 of culture (P< 0.001). Immunocytochemistry using CD73 and CD146 did not show consistent results. Flow cytometry analysis using CD73 and CD146 showed varied expression between passages. CONCLUSION: Cells isolated from the pulp of deciduous teeth and permanent teeth are a viable source of stem cells.
| Item Type: | Thesis (Masters) |
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| Uncontrolled Keywords: | stem cells ; immunocytochemistry ; flow cytometry ; deciduous teeth ; permanent teeth ; CD73 ; CD146 ; phenotype. |
| Subjects: | DENTAL > Oral Pathology and Microbiology |
| Depositing User: | Subramani R |
| Date Deposited: | 12 Nov 2017 13:52 |
| Last Modified: | 12 Nov 2017 13:52 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/3908 |
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