Ramasamy,, N (2009) Studies on the Leaves of Punica Granatum Linn. and its Invitro Inhibition of Ache for Potential Use in Alzheimer’s Treatment. Masters thesis, College of Pharmacy, Madurai Medical College, Madurai.
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Abstract
This dissertation covers the pharmacognostic parameters of the leaves of Punica granatum, Var. Ganesh belonging to the family: Punicaceae, such as macroscopical, microscopical including powder analysis and physical standards like ash values, extractive values, etc have been studied and presented. Microscopical studies reveals the presence of calcium oxalate prismatic crystals, druses in the mesophyll region and uniform parallel striations in the upper epidermis along with usual leaf tissues. The preliminary phytochemical studies reveals the presence of alkaloid, carbohydrate, glycoside, phytosterol, tannins, proteins, flavonoids, terpenoids in leaf powder. TLC of the chloroform, acetone and ethanol extracts of leaves of P.granatum shows the presence of ursolic acid. The percentage of ursolic acid in chloroform, acetone, ethanol extracts were determined by HPLC as 20.34, 0.32, 0.15 respectively. The commercial exploitation of ursolic acid is recommended. Acute toxicological study In the present study it was confirmed that the chloroform extract was nontoxic and the LC50 value was 2100 ppm and it need further evaluation in animal model by acute & chronic toxicological studies. Preliminary invitro AChE inhibitory activity The Ellman method for the Acetyl cholinesterase assay was standardized and validated. The preliminary observation of the various extracts of leaves of P.granatum by invitro study showed significant AChE inhibitory activity in the following order in a dose dependent manner . Chloroform extract > ethanol extract > acetone extract. The IC50 value for chloroform acetone, ethanol extracts were 1.8, 2.1, 1.8mM respectively. It is comparable with the standard drug tacrine. It is concluded that it can be optimistic that the present investigation present a herbal drug of a safe and potential chemotherapeutic target in the treatment of Alzheimer’s disease and commercial exploitation of ursolic acid extraction. AChE inhibitors, which enhance cholinergic transmission by reducing the enzymatic degradation of acetylcholine, are the only source of compound currently approved for the treatment of Alzheimer’s Disease (AD) by U.S. Food and Drug Administration (USFDA) and European medicines Agency (EMEA).
| Item Type: | Thesis (Masters) |
|---|---|
| Uncontrolled Keywords: | Punica Granatum Linn; Invitro Inhibition; Alzheimer’s Treatment |
| Subjects: | PHARMACY > Pharmacognosy |
| Depositing User: | Ravindran C |
| Date Deposited: | 05 Oct 2017 05:26 |
| Last Modified: | 06 Oct 2017 03:30 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/3561 |
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