Suribabu Mummidi, V V (2009) Quantitative Analysis of Gallic Acid and Ellagic Acid in Triphala Churnam Tablet Formulation by Rp-Hplc. Masters thesis, J K K Nataraja College of Pharmacy, Komarapalayam.
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Abstract
Ayurveda, the ancient system of herbal medicine is growing popular worldwide, but a number of factors such as standardization and stability studies are still at an infant stage for an ayurvedic product. The drug analysis plays an important role in the development of drugs, their manufacture and therapeutic use. These drugs may be in single component or multi-component dosage forms. The multi-component dosage forms prove to be effective due to the combined mode of action on the body. The complexity of dosage forms including multiple drug entities possesses considerable challenge to the analytical chemist during the development of assay procedures for ayurvedic formulations. For the estimation of drugs present in multi-component ayurvedic formulations, chromatographic methods are considered to be more suitable than spectrophotome tric methods due to its very high sensitivity. The present study deals with the development of new RP-HPLC method for the estimation of gallic acid and ellagic acid in the triphala churnam tablet formulation and then validation of the method as per ICH guidelines (International Conference on Harmonization of Technical Requirement for Registration of Pharmaceuticals for Human use; Plan: Study the physicochemical properties of drug (pH, solubility and molecular weight, selection of chromatographic conditions (mobile phase, column, flow rate, etc.), Optimization of the method, Study of system suitability parameters, validation of proposed method and applying developed method to marketed formulation. For gallic acid: A simple reverse phase high performance liquid chromatographic method exploying symmetry shield C-18 column has been developed for analysis of gallic acid. Good separation was achieved by employing an isocratic system using acetonitrile and 0.01 % phosphate buffer. The detection was carried out at 270 nm. The method was validated for linearity, accuracy, precision, robustness and ruggedness. The elution was carried out with a mixture of acetonitrile and phosphate buffer pH adjusted to 4.5 and the ratio 80: 20 at a constant flow rate 1 ml/min. The analytical wavelength was 270 nm. The developed HPLC method is simple, accurate and reproducible the following advantages. It uses simple symmetry shield C-18 column under less consumption of the mobile phase consist of acetonitrile and phosphate buffer (pH 4.5) and also less run time. So this method can be used as a routine analytical tool for the analysis of gallic acid in herbal formulations. In the present study the gallic acid content of 3.06 mg per tablet was observed in triphala churnam tablet formulation. For ellagic acid : A simple reverse phase high performance liquid chromatographic method exploying symmetry shield C-18 column has been developed for analysis of ellagic acid. Good separation was achieved by employing an isocratic system using acetonitrile and 0.01 % phosphate buffer. The detection was carried out at 254 nm. The method was validated for linearity, accuracy, precision, robustness and ruggedness. The elution was carried out with a mixture of acetonitrile and phosphate buffer pH adjusted to 4.5 and the ratio 80: 20 at a constant flow rate 1 ml/min. The analytical wavelength was 254 nm. The developed HPLC method is simple, accurate and reproducible the following advantages. It uses simple symmetry shield C-18 column under the less consumption of mobile phase consist of acetonitrile and phosphate buffer (pH 4.5) and also less run time. So this method can be used as a routine analytical tool for the analysis of ellagic acid in herbal formulations. In the present study the ellagic acid content of 0.955 mg per tablet was observed in triphala churnam tablet formulation.
| Item Type: | Thesis (Masters) |
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| Uncontrolled Keywords: | Ayurveda; Chromatographic methods; Galli; Ellagic acid; Triphala Churnam |
| Subjects: | PHARMACY > Pharmaceutical Analysis |
| Depositing User: | Ravindran C |
| Date Deposited: | 09 Aug 2017 04:52 |
| Last Modified: | 09 Aug 2017 05:47 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/2584 |
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