Sri Bharathi, G S (2021) Evaluation of Methanolic Leaf Extract of Cymbopogon Citratus on Alcohol Withdrawal Syndrome in Wistar Rats. Masters thesis, Nandha College of Pharmacy, Erode.
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Abstract
Alcohol withdrawal syndrome refers to a set of symptoms that occurs within few hours following the cessation of alcohol. Some of the symptoms are anxiety, tremor, hyper locomotion, sweating, nausea, vomiting, and agitation. On severe cases, it may lead to Delirium tremens and Seizures. Benzodiazepines are the popular class of drugs used in the treatment of AWS. But on prolonged usage it may also cause addiction. So, in mild to moderate symptoms of AWS herbal medicines can be used to suppress those symptoms which is of less or no side effect. Wistar albino rats were used in this study. For which permission was granted from the Institutional Animal Ethics Committee[NCP/IAEC/2021-22/01]. Cymbopogon citratus, commonly known as lemongrass is a perennial herb with therapeutic activities such as anti-obesity, anti-diabetic, anti-hepatotoxic, anti-anxiety and anti-microbial activity. It is selected to evaluate its activity against AWS. The plant was identified and authenticated by the Tamilnadu Agricultural University, Coimbatore[BSI/SRC/5/23/2020/Tech]. The leaves of the plant were collected and the methanolic extract was prepared by maceration process. Phytochemical screening confirmed the presence of flavonoid, aldehyde, terpenoid, tannin, phenolic compounds, saponin, alkaloid, unsaturated steroids and steroid derivatives. Alcohol withdrawal syndrome was induced in the wistar rats by administering 10% ethanol for 28 days followed by withdrawal. Diazepam(2mg/kg ip.,) was used as a standard in this study. MECC 200mg/kg and MECC 400 mg/kg were administered to group IV and group V respectively from day 22 to 28. The behavioral parameters were assessed using apparatus such as open field, rota rod, elevated plus maze, forced swim test on the 29th day. The ethanol withdrawal groups shown anxiety, muscle incoordination, depression and hyper locomotion. Which was attenuated by diazepam. But diazepam administration produced sedative effects. Administration of the MECC produced results similar to that of normal control. Then blood was collected on the 31st day and the biochemical parameters such as AST, ALT and ALP were assessed in the serum. The withdrawal group shown increased level of these biomarkers due to hepatotoxicity. Diazepam further increased the hepatic toxicity thus showing increased level of these biomarkers. MECC administration decreased the level of these biomarkers. Animals were sacrificed on the 31st day and the brain and liver were isolated. The brain and liver homogenates were prepared and MDA, SOD, CAT, GSH, GPx were measured. The withdrawal group shown decrease in the level of these antioxidants. Diazepam administration increased the level of these antioxidants in brain. But, in liver it further decreased the level of antioxidants when compared to that of the withdrawal group. MECC (200mg/kg and 400mg/kg) produced significant increase in the level of these antioxidants. The brain and liver samples were histopathologically analysed. The histopathology reports also supports the therapeutic and protective effects of Cymbopogon citratus. CONCLUSION: Alcohol withdrawal syndrome is a group of symptoms that occurs following the cessation of alcohol after a period of excessive alcohol use. These symptoms occur due to alcohol-induced chemical imbalances in the brain which result in excessive neuronal activity if the alcohol is withheld. Herbal medicines can be used for a very long period of time to treat them without possessing any side effects. Based on evidence from previous studies, Cymbopogon citratus was chosen and evaluated for its efficacy against Alcohol withdrawal syndrome in Wistar rats. From the biochemical and behavioral results it can be concluded that the methanolic leaf extract of Cymbopogon citratus possess anti-oxidant activity and it also attenuates the symptoms of Alcohol Withdrawal Syndrome in Wistar rats.
| Item Type: | Thesis (Masters) |
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| Additional Information: | 261925403 |
| Uncontrolled Keywords: | Evaluation, Methanolic Leaf Extract, Cymbopogon Citratus, Alcohol Withdrawal Syndrome, Wistar Rats. |
| Subjects: | PHARMACY > Pharmacology |
| Depositing User: | Subramani R |
| Date Deposited: | 03 Dec 2022 13:04 |
| Last Modified: | 03 Dec 2022 13:04 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/21026 |
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