Pavithra, M (2022) Expression of Cytokeratin 17 (CK17) in Oral Squamous Cell Carcinoma and Normal Mucosa. Masters thesis, Ragas Dental College and Hospital, Chennai.
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Abstract
BACKGROUND: The most common cancer worldwide is head and neck cancer. Oral cancer is the 11th most common cancer in the world. Oral squamous cell carcinoma (OSCC) is the second most common cancer reported in India. OSCC is the most common malignancy and represents more than 90% of all head and neck cancers. More than 640,000 new cases are reported worldwide annually, with high rate of morbidity and mortality of >50%. According to recent Globacon – 2018 data 130,000 new cases of OSCC are detected every year in India. The reason for such high prevalence in India is primarily tobacco consumed in form of gutka, snuff, betel quid and misri. Cytokeratins (CKs) are a group of Intermediate Filament proteins in the epithelium comprising a heterodimer of an acidic and a basic keratin commonly called keratin pair. According to Moll et al, there are 19 subclasses of CK, which are classified based on their molecular weights. CKs are place specific and may change when growth rate rises or when degree of differentiation is altered pathologically. Among the Cytokeratins, CK 17 immunoexpression increases as the grade of epithelial dysplasia advances. CK17 expression increase the cell mobility and migration, indicating that it may lead to architectural alterations in dysplastic epithelia and carcinoma. In many studies, compared to normal, CK 17 is overexpressed in squamous cell carcinoma of oral cavity, cervix, larynx, oesophagus and lungs. The present study is designed with an aim to correlate Cytokeratin 17 expression by immunohistochemistry with histological grade of Oral Squamous Cell Carcinoma which predicts aggressiveness of the tumor and thus helps in early diagnosis and treatment. AIM OF THE STUDY: To study the expression of Cytokeratin 17 in normal oral mucosa and different grades of Oral Squamous Cell Carcinoma. OBJECTIVES: 1. To evaluate expression of Cytokeratin 17 on formalin fixed paraffin-embedded tissue sections of normal oral mucosa using immunohistochemistry. 2. To evaluate expression of Cytokeratin 17 on formalin fixed paraffin-embedded tissue sections of Oral Squamous Cell Carcinoma using immunohistochemistry. 3. To compare the expression of Cytokeratin 17 on formalin fixed paraffin-embedded tissue sections of normal oral mucosa and Oral Squamous Cell Carcinoma using immunochemistry. MATERIALS AND METHODS: In this study, the population comprised of 55 (N=55) formalin fixed, paraffin embedded tissue specimens. The samples were divided into 4 groups namely: Group I (oral normal mucosa), Group II (well differentiated OSCC), Group III (moderately differentiated OSCC) and Group IV (poorly differentiated OSCC). CK17 expression was done using immunohistochemistry and the sections were evaluated for tissue localization, cellular localization, nature and intensity of the staining. P < 0.05 was considered significant. RESULTS: Overall 98% of the samples showed with CK 17 expression in the superficial epithelium. A gradual decrease in intensity of staining of suprabasal layer was observed with increase in grades of OSCC. Interestingly, in our study, all the cases expressed in both suprabasal and basal layer having the staining intensity 100% diffuse. While comparing grades of OSCC, 67% of the poorly differentiated OSCC samples showed complete loss of CK 17 staining in malignant tumor islands and 73% of well differentiated OSCC samples showed intense staining of central keratotic foci of keratin pearls. CONCLUSION: Based on the data obtained, CK17 has a potential diagnostic marker of OSCC that are under high risk for malignant transformation.
| Item Type: | Thesis (Masters) |
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| Additional Information: | Reg.No.241921153 |
| Uncontrolled Keywords: | Oral Squamous Cell Carcinoma, immunohistochemistry, CK17, diffuse, intense, suprabasal, basal layers. |
| Subjects: | DENTAL > Oral Pathology and Microbiology |
| Depositing User: | Subramani R |
| Date Deposited: | 08 May 2022 09:22 |
| Last Modified: | 23 Mar 2024 07:33 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/19792 |
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