Mumtaj, J (2019) Formulation and Evaluation of Doxorubicin Nanosponges. Masters thesis, RVS College of Pharmaceutical Sciences, Coimbatore.
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Abstract
Nanosponges are microscopic particles with few nanometers wide cavities, in which a large variety of substances can be encapsulated. These particles possess the ability to carry both lipophilic and hydrophilic substances and thereby improving the solubility of poorly water soluble molecules. Drugs encapsulated within the nanosponge pores are shielded from premature destruction and stability of drug is enhanced. Main objective of this study was to formulate doxorubicin loaded nanosponges using polymer to target cancer cells (breast cancer, colorectal cancer or oesophageal cancer) and release the drug in a controlled manner. This formulation reduced the side effects, minimized the dosing frequency and dose. The present work aimed at formulating doxorubicin nanosponges with polymer name hydrophobic polymer using emulsion solvent diffusion method. This method was simple and cost effective. Preformulation studies were carried out to find out the solubility of doxorubicin. Solubility test gave an idea that doxorubicin is water soluble and soluble in solvents like acetone, dichloromethane etc. FTIR and UV spectral studies authenticate the spectra obtained with the sample drug matched with standard pure drug. UV spectra gave the maximum absorption peak at 232nm. The comparison of FTIR spectra of doxorubicin and mixture of doxorubicin and polymer confirms that there is no appearance of additional new peaks and disappearance of existing peaks from that of the drug. This indicates that there is no interaction between the drug and polymer used in the study. Formulation was carried out by emulsion solvent diffusion method. Trial batches indicated that hydrophilic polymers are not suitable for the doxorubicin nanosponges. The hydrophilic polymers produced no yield or very less yield. Hydrophobic polymers produced good formulations.eudragit were selected for further studies. Scanning electron micrograph of the prepared nanosponges at different magnification showed that the nanosponges were porous with a smooth surface morphology and spherical shape. The spongy and porous nature of nanosponges was clearly observed in the SEM images. Particle size and zeta potential was determined by Malvern Zeta sizer. The particle size analysis confirmed that the prepared sample were in the nanometer range. Average particle size obtained for the formulations F9 is 1.708e4. Zeta potential values of nanosponges indicated that the formulated nanosponges are stable. The amount of drug being entrapped in nanosponges was calculated and all the prepared nanosponges were found to possess very high entrapment efficiency. From the in-vitro release data from the dialysis bag diffusion method it was found that formulations F1 to F5 & F6 to F10 showed the best release of 89.90, 88.79, 81.75, 72.86, 67.56 and 90.18, 87.10, 77.94, 70.14, 69.86 respectively at the end of 48 hours. Increase of drug release was observed as a function of drug: polymer ratio. It was observed that the drug release decreased with an increase in the amount of polymer for each formulation. This is because the newly developed nanosponges is believed to exhibit a core shell structure with a hydrophobic core formed by eudragit and a hydrophilic shell formed by PVA macromolecules. CONCLUSION: The doxorubicin nanosponges can be formulated by cost effective and easy emulsion solvent diffusion method using hydrophobic polymers such as eudragit. The formulated doxorubicin nanosponges can be used in the treatment of breast cancer. This can be targeted to the cancer cells and produce sustained drug delivery which in turn reduces the dose, frequency of administration and the side effects.
| Item Type: | Thesis (Masters) |
|---|---|
| Additional Information: | 261710759 |
| Uncontrolled Keywords: | Formulation, Evaluation, Doxorubicin, Nanosponges. |
| Subjects: | PHARMACY > Pharmaceutics |
| Depositing User: | Thavamani K |
| Date Deposited: | 30 Mar 2022 12:48 |
| Last Modified: | 30 Mar 2022 12:50 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/19117 |
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