Kevin Martin, M P (2023) Study on Virulence Factors and Antimicrobial Susceptibility Pattern of Non Urinary Isolates of Escherichia Coli With Special Refefernce to Molecular Characterization of Carbapenem Resistant Isolates for blaNDM and blaVIM. Masters thesis, Madras Medical College, Chennai.
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2004 001 23 Kevin Martin M P.pdf Download (2MB) | Preview |
Abstract
INTRODUCTION AND OBJECTIVES Escherichia coli are facultative pathogen, that are part of the normal human intestinal flora. However, when it enters into unnatural sites, they cause various infections. Urinary tract infection, wound infection, sepsis, pneumonia in immunosuppressed hospitalized patients, and neonatal meningitis are the common infections caused by E. coli. The ability of E. coli to cause extraintestinal infections depends on virulence factors, which help in the survival of E. coli under adverse conditions present in those sites. Along with virulence factors, they have developed resistance to numerous antimicrobial agents due to the production of enzymes like Extended spectrum β-lactamase(ESBL). Nowadays resistance to carbapenems has become more prevalent due to production of carbapenemases. Hence this study was conducted to detect virulence factors and antimicrobial susceptibility patterns of non urinary isolates of Escherichia coli including ESBL production and PCR was performed to detect the blaNDM and blaVIM gene in carbapenem resistant E. coli isolates. METHODS A total of 200 Escherichia coli isolates were obtained from various clinical samples received at the Microbiology laboratory, RGGGH. These isolates were tested for virulence factors such as hemolysin, serum resistance, biofilm and gelatinase. Isolates were also tested for Antimicrobial Susceptibility Pattern including Extended spectrum beta-lactamases(ESBL) production and PCR was performed to detect the blaNDM and blaVIM gene in carbapenem resistant E. coli isolates. RESULTS Among 200 isolates of E. coli, 45(22.5%) of them were hemolytic, 81(40.5%) isolates showed serum resistance, 37(18.5%) were positive for biofilm and 78(39%) were positive for gelatinase. Antibiotic susceptibility pattern showed that maximum isolates were sensitive to meropenem and piperacillin/tazobactam. 54% of the isolates were ESBL producer. Out of 19 carbapenem resistance E. coli isolates, NDM was detected in eight(42.1%) isolates, whereas the VIM gene was detected in one(5.2%) isolate. One(5.2%) isolate were positive for both blaNDM and blaVIM genes. CONCLUSION The present study showed that E. coli cause various infections including wound infections, abscess, VAP and septicaemia. They secrete numerous virulence factors which enable them to adhere, colonize and cause damage to different tissues establishing infection and also exhibit multi drug resistance by producing ESBL. Carbapenem resistance among Gram negative bacteria has now become a worldwide concern. Therefore, detection of NDM and VIM like carbapenemases will help in the early management and prevention of spread of such resistant isolates in hospital and community which in turn will stop the spread of MDR strains in the community.
| Item Type: | Thesis (Masters) |
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| Additional Information: | Reg No: 200420100504 |
| Uncontrolled Keywords: | Virulence Factors, Antimicrobial Susceptibility Pattern, Non Urinary Isolates, Escherichia Coli, Special Reference,o Molecular Characterization, Carbapenem Resistant Isolates, blaNDM, blaVIM. |
| Subjects: | MEDICAL > Microbiology > MEDICAL > Microbiology |
| Depositing User: | Subramani R |
| Date Deposited: | 30 Apr 2021 15:38 |
| Last Modified: | 05 May 2024 01:24 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/15381 |
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