Kalpana, K (2020) Evaluation of Collagen Fibers in Oral Leukoplakia, Oral Submucous Fibrosis and Oral Squamous Cell Carcinoma using Picrosirius Red by Polarizing Microscopy. Masters thesis, Tamil Nadu Government Dental College and Hospital, Chennai.
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Abstract
BACKGROUND: In India oral squamous cell carcinoma is a common malignancy accounting for 50-70% of total cancer mortality. Oral squamous cell carcinoma arise either de novo or from oral potentially malignant disorders mainly leukoplakia. AIM OF THE STUDY: The aim of the study is to evaluate collagen fibers in oral leukoplakia, oral submucous fibrosis and oral squamous cell carcinoma using picrosirius red by polarizing microscopy. OBJECTIVE: The objective is to determine the predictive value of collagen in oral leukoplakia, oral submucous fibrosis, and oral squamous cell carcinoma. MATERIALS AND METHOD: The study included 15 cases of well differentiated squamous cell carcinoma, 15 cases of oral submucous fibrosis without malignant transformation, and 15 cases of Leukoplakia (clinically) with mild to severe dysplasia. The birefringence color of collagen is evaluated using picrosirius red & polarizing microscopy. RESULTS: In case of well differentiated squamous cell carcinoma, greenish yellow color is observed in 10 cases (66.7%), greenish orange color is observed in 3 cases (20%), and no significant color in 2 cases (13.3%). In case of oral submucous fibrosis, greenish yellow color is observed in 5 cases (33.3%), green/green-orange color is observed in 4 cases (26.6%), and greenish orange color is observed in 6 cases (40%). In case of Leukoplakia, greenish yellow is observed in 11 cases (73.3%), reddish orange is observed in one case (6.6%), greenish orange in one case (6.6%), and no significant color is observed in 2 cases (13.3%). CONCLUSION: In India tobacco chewing habit is more prevalent causing oral squamous cell carcinoma and potentially malignant disorders. Most of the oral squamous cell carcinoma will clinically appear as leukoplakia or erythroplakia. None of the treatment is effective in preventing the malignant transformation from oral potentially malignant disorders. Epithelial dysplasia is one the main indicators for malignant transformation. Before dysplastic changes there are few evidence of change in the extracellular matrix mainly collagen. Even though many traditional stains are available, picrosirius red is the best one for collagen. Picrosirius red is the special stain for collagen as it stains even the thin fibers. Under polarizing microscopy green or greenish yellow color indicates loosely packed fibers, whereas reddish orange, greenish orange, and yellowish orange indicates tightly packed collagen fibers. In case of well differentiated squamous cell carcinoma and leukoplakia greenish yellow color is predominant [loosely packed fibers]. In case of oral submucous fibrosis greenish orange was predominant [tightly packed fibers]. Further studies on large sample size with follow up of the patient are necessary to find the biological behavior of the lesion.
| Item Type: | Thesis (Masters) |
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| Additional Information: | 24171002 |
| Uncontrolled Keywords: | collagen fibers, picrosirius red, polarizing microscopy, well differentiated squamous cell carcinoma, oral submucous fibrosis, and leukoplakia. |
| Subjects: | DENTAL > Oral Pathology and Microbiology |
| Depositing User: | Subramani R |
| Date Deposited: | 24 Feb 2021 16:16 |
| Last Modified: | 26 Feb 2021 00:56 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/14475 |
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