Rachel, J (2020) Morphology and Distribution of Langerhans cells in Normal Nasal Mucosa and Nasal Polyps. Masters thesis, Christian Medical College, Vellore.
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Abstract
BACKGROUND: Nasal polyps are outgrowths of nasal mucosa in 4% of the population. Nasal mucosa is repeatedly bombarded by exogenous antigens. Langerhans cells (LCs) being members of the dendritic cell family reside in the nasal mucosa and are responsible for maintaining the immunological environment. AIM OF THE STUDY: To study the morphology and distribution of LCs in normal nasal mucosa and compare the same with nasal polyps by zinc iodide-osmium (ZIO) technique and immunohistochemistry. OBJECTIVES: 1. To study the morphology of ZIO and CD1a positive LCs in nasal mucosa of controls and cases by histochemistry and by immunohistochemistry respectively. 2. To count and compare the number of ZIO and CD1a positive LCs per unit area of lamina propria in nasal mucosa of controls and in cases by histochemistry and by immunohistochemistry respectively. 3. To quantify the number of eosinophils per unit area of lamina propria in controls and in cases by routine H&E staining. 4. To study the correlation between the number of ZIO and CD1a positive LCs and eosinophils in controls and in cases. MATERIALS AND METHODS: Ethical approval for the study was obtained from the Institutional Review Board of Christian Medical College, Vellore. The morphology and distribution of LCs in controls and cases was demonstrated by two methods, the histochemical method using zinc iodide osmium staining and immunohistochemistry method using CD1a antibody. Normal nasal mucosa (control) tissues were obtained from 21 patients undergoing septoplasty. The nasal polyp specimens (cases) were obtained from 23 patients undergoing excision biopsy from the Department of ENT, Christian Medical College, Vellore. In addition to this, 27 more cases were collected for routine H&E staining for counting eosinophils. Informed consent was obtained from all the patients. Data was entered in Epidata (version 3.1) software and data analysis was done using SPSS version 24. • Descriptive statistics namely range, mean with standard deviation, median with interquartile range were obtained for continuous variables. • Non-parametric Mann Whitney U test was done to determine the significance in the number of ZIO and CD1a positive LCs and also eosinophils between cases and controls. • To determine the correlation between the number of ZIO and CD1a positive LCs and also eosinophils in controls and cases with nasal polyps, Pearson’s correlation test was done. RESULTS: On comparing the distribution of ZIO/ CD1a positive LCs across cases and controls, they were significantly higher in cases. The number of eosinophils were also significantly higher in cases when compared with controls. On comparing the distribution of ZIO/CD1a positive LCs between fungal and non-fungal nasal polyps (cases), they were statistically insignificant. On comparing the distribution of eosinophils between fungal and non-fungal nasal polyps (cases), they were statistically insignificant. There was no correlation between ZIO/ CD1a positive LCs and eosinophils in cases and controls.
| Item Type: | Thesis (Masters) |
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| Additional Information: | 201733202 |
| Uncontrolled Keywords: | Morphology and Distribution of Langerhans cells in Normal Nasal Mucosa and Nasal Polyps |
| Subjects: | MEDICAL > Anatomy |
| Depositing User: | Subramani R |
| Date Deposited: | 06 Feb 2021 04:09 |
| Last Modified: | 06 Feb 2021 04:09 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/13917 |
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