Immunohistochemical detection of 8-hydroxy deoxyguanosine (8-OHdG): A Biomarker of oxidative DNA damage in oral submucous fibrosis

Chandramohan, K (2018) Immunohistochemical detection of 8-hydroxy deoxyguanosine (8-OHdG): A Biomarker of oxidative DNA damage in oral submucous fibrosis. Masters thesis, Adhiparasakthi Dental College and Hospital, Melmaruvathur.


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BACKGROUND: Oral submucous fibrosis (OSMF) is one of the common potentially malignant disorder prevailing in India. The primary etiological factors include tobacco and are canut which contain numerous Reactive Oxygen Species (ROS). The imbalance between ROS and antioxidant status creates oxidative stress. Reactive oxygen species attack guanine bases in DNA and form 8-Hydroxy Deoxyguanosine (8-OHdG) which can be detected in patients who have diseases associated with oxidative stress. The oxidative DNA damage produced by oxidative stress may induce malignant transformation. The aim of the present study is to detect the expression of 8 -OHdG in OSMF and compare the expression within the different grades and normal oral mucosa. AIM OF THE STUDY: To study the Immunohistochemical expression of 8-OHdG in oral submucous fibrosis. MATERIALS AND METHODS: A total of 30 samples were examined for the immunohistochemical expression of 8-OHdG. The control group includes 10 formalin fixed paraffin embedded tissue blocks of normal buccal mucosa. The study group includes 20 cases of formalin fixed paraffin embedded tissue blocks of oral submucous fibrosis (5cases of very early, early, moderately advanced, advanced cases of OSMF). 3 micron thickness sections were made from each sample and stained with 8-OHdG antibody. The expression is scored and analysed statistically. The results were statistically analysed using Kruskal-Wallis and Mann - Whitney U test. RESULTS: Statistically significant difference exists in the intensity of 8-OHdG expression between the study groups. Increased intensity in the oral submucous fibrosis samples were observed when compared with normal mucosa. The p value obtained was less than 0.001 which was highly statistically significant. Staining intensity was also compared with different grades of OSMF, the p value obtained was less than 0.01 which was statistically significant. CONCLUSION: Our present study is the first attempt to evaluate the expression of 8-OHdG in tissue samples that revealed the role of free radicals and oxidative DNA damage which enhances the initiation and progression of Oral submucous fibrosis. Further research with a larger sample size, clinicopathologic correlation and long term followup will shed more light on the pathogenesis of Oral submucous fibrosis. It will also be useful for the development of new therapeutic stratergies targeting treatment modalities for the oral submucous fibrosis.

Item Type: Thesis (Masters)
Uncontrolled Keywords: Oral submucous fibrosis ; Oxidative DNA damage ; 8-OHdG ; Immunohistochemistry.
Subjects: DENTAL > Oral Pathology and Microbiology
Depositing User: Subramani R
Date Deposited: 14 Oct 2018 08:12
Last Modified: 14 Oct 2018 08:12

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