Neetu, Prince (2015) Investigation on Non-Fluorescent Fraction (NFF) of Aqueous Extract of Cleistanthus Collinus Leaves as an Antidote to Cleistanthus Poisoning. Masters thesis, Christian Medical College, Vellore.
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Abstract
INTRODUCTION: Cleistanthus collinus poisoning is common in rural south India. Water extract of Cleistanthus collinus leaves is a common suicidal poison. Victims consume either a boiled decoction (FLB, fresh leaves boiled) or fresh leaves blended and filtered. Mortality is about 28% and is stated to be higher with boiled decoction. AIM OF THE STUDY: The aim of the present study was to develop an antidote for Cleistanthus collinus poisoning OBJECTIVES: 1. To get the minimum lethal dose (MLD100) of FLB, FLG, purified Cleistanthin A and C. 2. To see if a particular fraction of Cleistanthus collinus extract (non fluorescent fraction of cold water extract, NFF) can prevent death in rats when administered after administering the lethal dose of boiled water extract / cleistanthin C. METHODS: Boiled decoction and fresh leaf ground extract was prepared and minimal lethal dose for the same was estimated. Toxic principles, Cleistanthin A and C was isolated to its single peak purity for experiments. Non fluorescent fraction (NFF) to try as an antidote was prepared from fresh leaf ice cold extract of C.collinus leaves. NFF was administered to the rats poisoned with boiled extract or cleistanthin C. Toxins were administered via oral gavage. RESULTS: Minimum lethal dose (MLD100) of FLB, FLG, purified Cleistanthin A and C in rats was determined. There is not much difference in MLD100 for FLB (75mg/100gm) and FLG (50mg/100gm) in rats. The concentration of Cleistanthin C in the MLD100 of FLB is sufficient to account for lethality of FLB. However, the concentrations of both Cleistanthin C and Cleistanthin A are insufficient to account for lethality in the case of FLG. Therefore we conclude that, the toxicity of fresh leaf ground is neither due to cleistanthin A or C, but due to a yet unidentified compound. Experiments with NFF as an antidote in C.collinus poisoning are yet to be standardized.
| Item Type: | Thesis (Masters) |
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| Uncontrolled Keywords: | Cleistanthus collinus ; Cleistanthin A ; Cleistanthin C ; Plant poisoning ; TLC ; HPLC. |
| Subjects: | MEDICAL > Physiology |
| Depositing User: | Punitha K |
| Date Deposited: | 24 May 2018 17:49 |
| Last Modified: | 27 May 2018 11:34 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/8075 |
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