Levels of Rankl and OPG In Gingival Crevicular Fluid during Orthodontic Tooth Movement

Ritika, Kailey (2011) Levels of Rankl and OPG In Gingival Crevicular Fluid during Orthodontic Tooth Movement. Masters thesis, Ragas Dental College & Hospital, Chennai.


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This study was undertaken to assess the change in RANKL and OPG levels in the GCF following the application of orthodontic force. GCF samples were collected from the upper and lower arches of orthodontic patients who had undergone upper and lower first bicuspid extractions. The samples were collected at two time points; T1-End of stage I (Leveling & aligning) and T2- Three weeks after application of retraction force. Disposable micropipettes were used for GCF collection and the collected samples were subjected to an ELISA test to determine the concentrations of RANKL and OPG. 1. Both upper and lower arches showed an increase in RANKL levels from T1 to T2. In the upper arch a statistically significant increase (p < 0.001) in the RANKL concentration was observed at T2 (1960 + 285pg/ml) compared to T1 (1520 + 247.3pg/ml). In the lower arch as well a statistically significant increase (p < 0.0001) in the RANKL concentration was observed at T2 (2008 + 276.7pg/ml) compared to T1 (1584 + 279.9 pg/ml) 2. A reciprocal decrease in the OPG concentration was observed in the upper and lower arches. The upper arch showed a decrease in OPG levels from 42.8 + 8.3 pg/ml at T1 to 29.6 + 8.43 pg/ml at T2. (p<0.0001). The lower arch showed a decrease in OPG levels from 40.35 + 7.472 pg/ml at T1 to 20.10 + 7.331 pg/ml at T2. (p<0.0001). 3. An increase in the relative RANKL/OPG ratio was seen in both arches. In the upper arch, RANKL/OPG ratio increased from 35.5 : 1 at T1 to 66.2 : 1 at T2. (p<0.01) The lower arch showed an increase in RANKL/OPG ratio from 39.3:1 at T1 to 99.1:1 at T2. (p<0.001). 4. Both factors, i.e, an increase in the RANKL levels and a decrease in the OPG levels contributed to the observed increase in RANKL/OPG ratio. This is in accordance with existing invivo studies by Mogi et al. and Bostanci et al. The present findings differed from a study done by Lu et al. where the OPG levels remained unchanged and only an increase in RANKL concentration contributed to the increase in RANKL/OPG ratio. 5. The study design varied from existing studies in the manner and duration of force application. Instead of elastic chains which were used in previous studies, this study used e-ties to apply the retraction force. This was done to provide a relatively constant force over the entire experimental period. Existing invivo studies have compared the cytokine levels 168 hours after application of orthodontic force. This study increased the duration of the force application to 3 weeks to obtain data belonging to the linear or acceleration phase of tooth movement which require an observation period of at least 15-21 days. 6. Further research in this field would help to refine the analysis of these biologic markers of tooth movement in a clinical setting. This sort of advancement would be especially useful in patients with bone metabolic problems as well as in patients suffering from inflammatory periodontal disease.

Item Type: Thesis (Masters)
Uncontrolled Keywords: Levels of Rankl and OPG ; Gingival Crevicular Fluid ; Orthodontic Tooth Movement.
Subjects: DENTAL > Orthodontics and Dentofacial Orthopedics
Depositing User: Kambaraman B
Date Deposited: 12 May 2018 08:41
Last Modified: 12 May 2018 08:41
URI: http://repository-tnmgrmu.ac.in/id/eprint/7720

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