Prabakaran, K (2016) Evaluation of Neuroprotective Role of Ethanol Extract of Pterocarpus Marsupium Roxb. Bark Against Monosodium Glutamate-Induced Excitotoxicity Model in SD Rats. Masters thesis, J.K.K. Nattraja College of Pharmacy, Komarapalayam.
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Abstract
The present study were As human life expectancy has increased, so too has the incidence of stress related neurodegenerative disorders such as AD, PD and HD. Plant extracts have a wide range of medicinal actions, and throughout history, they have been used to treat many different types of diseases. In the treatment of many diseases, antioxidant therapy plays a key role, so current research is now directed towards finding naturally occurring antioxidant of plant origin. Although medicinal herbs has been found to share many medicinal properties with ‘rasayans’ in Ayurveda, its neuroprotective effect in different experimental neurodegenerative models are still debatable. Actually, little is known about the feeding pattern and feeding rhythms that underly the hypophagia of the stress treated rats. The general behavior, anxiolytic, antidepressant and antioxidant enzyme examination of the rat whole brain, pattern of food and water intake are used for the first time, to assess the protective effect of ethanol extract of Pterocarpus marsupium bark (EEPM) against MSG-induced neurodegenerative injury. We anticipate that the behavioral tests used in the present study could contribute to the evaluation of potential drugs effective in the prevention of functional deficits induced by neurotoxic agent and may shed an insight into the mechanism of action. Hence, a special attention is focused to understand the treatment of neurodegenerative diseases by natural phenolic antioxidants from this plant. There are, indeed, a multitude of paradigms assessing various aspects of the behavioral performance and cognitive abilities. Till now, some of the paradigms will be not used at all in the evaluation of Pterocarpus marsupium bark against behavioral consequences of adult rats in stress. Hence, in order to contribute further to the knowledge on Pterocarpus marsupium bark, the objective of the present study has been designed to evaluate the possible neuroprotective effect of EEPM against MSG-induced excitotoxicity in adult rats. DISCUSSION: The present study was designed to evaluate the possible neuroprotective effect of ethanol extract of Pterocarpus marsupium Roxb. bark (EEPM) pre-treatment against the excitotoxic effect of MSG-induced neurodegeneration in SD rats. The neuroprotective effect was assessed using a series of general behavior (feed intake, water intake and body weight), anxiety and depression behavioral tests and biochemical analysis of the brain antioxidant elements in rat brain. Excitotoxicity was induced by intraperitoneal injection of MSG (2 g/kg) for seven days which resulted in glutamate receptor activation and Ca2+ overload (via a number of different mechanisms). To substantiate the neuroprotective effect of EEPM, the implications on general behavior, body weight (BW), food intake (FI) and water intake (WI) were also analyzed, as these behavior could be disturbed in depression and anxiety models that were employed in this study. Also there may be perturbations in the levels of brain antioxidant enzyme systems in stressful states. Hence, the effects of EEPM on the levels of superoxide dismutase (SOD), Catalase (CAT), glutathione (GSH), and lipid peroxidation (LPO) were studied in the whole rat brain. SUMMARY AND CONCLUSION: The study was undertaken to evaluate the probable neuroprotective effect of pre-treatment of ethanol extract of Pterocarpus marsupium Roxb. bark (EEPM) against monosodium glutamate-induced excitotoxicity model in SD rats. The neuroprotective effect was assessed by measuring the changes in water intake, feed intake and body weight changes in addition to using a sequence of behavioral tests (anxiety and depression) and biochemical analysis (SOD, CAT, GSH and LPO) of the rat brain. The protective effect of EEPM was evaluated for depression using forced swim test and anxiety using actophotometer and elevated plus-maze apparatus in MSG induced models. To substantiate the neuroprotective effect of EEPM and its implications on general behaviors viz., feed intake (FI), water intake (WI) and body weight (BW) were also undertaken in MSG induced models, as these behavior could be profoundly disturbed in anxiety, depression and may impair memory and cognitive abilities. Also there may be perturbations in the levels of brain antioxidant enzyme system in stressful states. Hence, the effects of EEPM on the levels of SOD, CAT, GSH and LPO were studied in the rat brains. Administration of MSG for 7 days resulted in elevated levels of anxiety and depression. Pretreatment with EEPM for 30 days prevented the effect of MSG in rats. The anxiety and depression parameters were attenuated significantly in MSG treated rats. Moreover, the changes in food intake, water intake and body weight were also minimizes in MSG treated rats. The perturbations in the levels of SOD, CAT, GSH & LPO were also found to be attenuated in EEPM groups.
| Item Type: | Thesis (Masters) |
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| Uncontrolled Keywords: | Neuroprotective Role ; Ethanol Extract ; Pterocarpus Marsupium Roxb ; Bark ;t Monosodium Glutamate ; Excitotoxicity Model ; SD Rats |
| Subjects: | PHARMACY > Pharmacology |
| Depositing User: | Ravindran C |
| Date Deposited: | 29 Dec 2017 07:53 |
| Last Modified: | 29 Dec 2017 07:53 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/4950 |
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