Sivasubramanian, J (2013) Evaluation of Gingival Crevicular Fluid and Saliva as Comparative Markers in the Expression of Interleukin -1 β during orthodontic Tooth movement: A In Vivo study. Masters thesis, Ragas Dental College and Hospital, Chennai.
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Abstract
OBJECTIVE: This study was undertaken to correlate the findings of presence of Interleukin - 1β in GCF and saliva during initial levelling stage of orthodontic treatment and to substantiate if saliva as a medium could be used as a suitable marker for establishing the expression of IL - 1β. MATERIALS AND METHODS: GCF and Whole Saliva samples were collected from ten orthodontic patients (6 females and 4 males, aged 15 - 25 yrs) who had Little’s Irregularity Index score ( 10) referring to very severe irregularity and had undergone upper and lower first bicuspid extractions. The samples were collected at the following stages:- T 0 – Pre Treatment, T 1 – 7 days after initiation of orthodontic treatment, T 2 - 30 days after initiation of orthodontic treatment. Disposable micropipettes were used for GCF collection. The saliva samples were collected in sterile containers. The collected samples were subjected to an ELISA test to determine the concentrations of IL - 1 β. RESULTS: The results of our study showed that IL - 1β levels were highly increased on the 7th day in both GCF and Saliva (GCF - 288.57 +/- 111.88 Pg/ml, Saliva - 272.50 +/- 108.16 Pg/ml) after appliance activation when compared to the baseline levels (GCF - 151.20 +/- 129.21 Pg/ml, Saliva - 186.30 +/- 163.57 Pg/ml), , and they gradually decreased on the 30th day (GCF - 228.67 +/- 103.37 Pg/ml, Saliva - 211.75 +/- 120.09 Pg/ml) when compared to the realtively increased levels seen on 7th day. No statistically significant results could be drawn because of high amount of inter individual variability. CONCLUSION: Results of the current study, show a positive indication that saliva could be used as a viable substitute for GCF when used for assessing bone remodelling for patients undergoing orthodontic treatment.
| Item Type: | Thesis (Masters) |
|---|---|
| Uncontrolled Keywords: | Orthodontic tooth movement ; Little’s Irregularity Index ; Gingival crevicular fluid ; Whole Saliva ; Interleukin-1β. |
| Subjects: | DENTAL > Orthodontics and Dentofacial Orthopedics |
| Depositing User: | Subramani R |
| Date Deposited: | 12 Nov 2017 15:27 |
| Last Modified: | 12 Nov 2017 15:27 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/3912 |
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