Arun, R (2013) A Comparative evaluation in different types of treatments on Titanium alloy samples with or without Gentamicin and its effect on Biofilm - An In Vitro study. Masters thesis, Sree Mookambika Institute of Dental Sciences, Kulasekharam.
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Abstract
INTRODUCTION : Use of Osseointegrated oral implants has been an excellent method for replacement of missing teeth. Biofilm formation on oral implants can cause inflammation of peri-implant tissues, which can affect the long term success of Osseointegrated implants. After exposure of implant to oral cavity, an acquired pellicle formed from salivary biopolymers becomes adsorbed to the implant surface. This pellicle forms the interface between the implant surface and initial microorganism like the Streptococcus mitis, Streptococcus sanguis and Streptococcus oralis. These bacterial microorganisms create a precondition for adhesion of periodontal pathogens such as Haemophillus Actinomycetemcomitans, Porphyromonas Gingivalis, Prevotella intermedia, Treponema Denticola which can induce peri implantitis with characteristic inflammation of the peri implant mucosa and destruction of the peri implant bone AIMS & OBJECTIVES : To evaluate biofilm formation on various surface treated implants . 1. Comparative evaluation of Biofilm formation amongst 5 differently treated surface on Titanium samples. 2. To evaluate the difference in the delay of biofilm formation amongst various surface. METHODOLOGY : Six set of polished Titanium samples was blasted with sintered HA and TiO2. Another set of samples were blasted and later loaded with Gentamicin drug by vaccum drying. All the samples are sterilized by autoclaving. The control group were plain polished and gentamicin drug loaded samples. All the samples were autoclaved and evaluation of the strains was done for biofilm. Bacterial adhesion was evaluated on time intervals of 0 hr,1 hr, 4 hrs 24 hrs and 48 hrs. Bio film was evaluated in colony forming units, and plotted against rough and polished samples. RESULTS : Analysis of variance ANOVA was the statistical tool employed to analyze the data. 1) All the samples showed bio film formation. 2) Bacterial adhesion was sequentially increasing in polished samples. 3) Initial bacterial adhesion was more on surface modified samples when compared to polished samples in the 1st hour 4) Bacterial adhesion was retarded in gentamicin coated HA blasted samples up to 24 hrs 5) Bacterial adhesion was considerably less on TiO2 blasted samples up to 48 hrs. SUMMARY : From the above findings it can be concluded that implant surface modified with TiO2 and gentamicin showed delayed biofilm formation even up to 48 hrs. These implants can retard the plaque formation thus prevents peri-implantitis in the primary healing stage. This in turn can prevent failure of implants. This is ideal in situations where the patient is having poor bone quality, poor oral hygiene and in patients suffering from debilitating disease. Surface modification with HA has gained considerable osteoconductive surface which is a boon for the production of future implants with less expense, however further studies are to be carried out to prove its efficacy.
| Item Type: | Thesis (Masters) |
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| Uncontrolled Keywords: | Titanium alloy samples ; with or without ; Gentamicin ; Biofilm ; In Vitro study. |
| Subjects: | DENTAL > Prosthodontics and Crown and Bridge |
| Depositing User: | Thavamani K |
| Date Deposited: | 11 Jul 2017 16:04 |
| Last Modified: | 12 Jul 2017 01:27 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/1487 |
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