Deepak, P (2020) To Evaluate the Effects of Antimicrobial Primer containing Selenium on Development of White Spot Lesion: An Invitro study. Masters thesis, Ragas Dental College and Hospital, Chennai.
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Abstract
AIM OF THE STUDY: The aim of the present study was to evaluate the effects of antimicrobial primer containing selenium on development of white spot lesion and also to evaluate shear bond strength after artificial caries induction. MATERIALS AND METHODS: Ninety human maxillary premolars atraumatically extracted for orthodontic purpose were used in this study. The samples were divided into two groups of 40 each, Group 1-Transbond XT primer (Etch + Transbond XT primer application +Transbond XT adhesive) Group 2- Denteshield TM primer (Etch + Denteshield primer +Transbond XT adhesive) remaining 10 samples in each group underwent only cross sectional micro-hardness test (at 10, 30,50,80,100 μm). After bonding all samples in both the groups were stored in artificial saliva solution for 24 hours. Of the 40 samples in each group 30 samples were subjected to microbial caries induction for 10 days. Microbial caries induction was performed by inoculating dental plaque containing streptococcus mutans in a medium of brain heart infusion broth which served as a nutritional medium for bacterial growth and acid production. The remaining 10 samples in each group were tested for only shear bond strength. After microbial caries induction for 10days, 10 samples from each group were tested for shear bond strength and cross sectional micro-hardness and remaining samples in each group were transferred to artificial saliva for a period of 30days and 60 days respectively (n=10) each. At the end of 30 days and 60 days the respective samples were evaluated for shear bond strength and cross sectional micro-hardness. Both the groups along with a control (ciprofloxacin), were evaluated for their antimicrobial property by agar diffusion test and minimum inhibitory concentration (MIC) was assessed. RESULTS: The results of cross sectional micro-hardness revealed that Group 2 showed reduced amount of mineral loss at all the time intervals when compared to Group 1. A statistically significant difference in the amount of mineral loss was noted between the groups after microbial caries induction for 10 days, 30days and 60 days. When assessing the shear bond strength between two groups, there was no statistical significant difference. The anti-microbial primer containing selenium Group 2 (Denteshield TM) provided effective antimicrobial property when studied by agar diffusion method. At a concentration of 60μg/ml the anti-microbial primer containing selenium Group 2 (Denteshield TM) provided effective antimicrobial property, assessed by minimum inhibitory concentration (MIC) using spectrophotometry. CONCLUSION: Within the limitations of this study it was concluded that the DenteshieldTM (selenium containing primer) inhibited biofilm and helped to reduce the white spot lesion without compromising the shear bond strength, and hence may be promising to reduce demineralization of enamel around the orthodontic brackets.
| Item Type: | Thesis (Masters) |
|---|---|
| Additional Information: | 241719151 |
| Uncontrolled Keywords: | Mineral loss, DenteshieldTM, Transbond XT, Microbial caries induction, Cross sectional microhardness, Shear bond strength, Agar diffusion test, Minimum inhibitory concentration (MIC). |
| Subjects: | DENTAL > Orthodontics and Dentofacial Orthopedics |
| Depositing User: | Subramani R |
| Date Deposited: | 24 Feb 2021 02:42 |
| Last Modified: | 24 Feb 2021 02:42 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/14447 |
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