Ramya, M (2020) Comparative Evaluation of Probiotic and Chlorhexidine Mouthwashes Effect on the Adhesion of Streptococcus Mutans on Stainless Steel Brackets: A Clinical Trial. Masters thesis, Tamil Nadu Government Dental College and Hospital, Chennai.
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Abstract
BACKGROUND: Mechanical debridement for plaque control during orthodontic therapy has lot of limitations. Hence it is thought that working on carious flora to inhibit them without any adverse effect on the patient is favourable for the patient. If this modality of plaque control is effective in orthodontic patients, it could result in large reduction of incidence caries and gingivitis during orthodontic treatment. AIM OF THE STUDY: To evaluate and compare the minimal adhesion of Streptococcus mutans over the stainless steel brackets between probiotic and chlorhexidine mouth washes. OBJECTIVES: To evaluate the adhesion of Streptococcus mutans on the stainless steel bracket for 30 days in patients using probiotic mouthwashes, evaluate the adhesion of Streptococcus mutans on the stainless steel bracket for 30 days in patients using chlorhexidine mouthwashes and compare the values obtained from RT-PCR. MATERIALS AND METHODS: The study population for this clinical case control study consisted of 30 individuals undergoing orthodontic therapy with stainless steel brackets. One group of patients (Group A) was given the chlorhexidine mouth wash, and the other group (Group B) was given the probiotic mouth wash. The patients were instructed on oral hygiene. Plaque samples were collected on the first day and after 4 weeks, suspended in 1 ml of sterile phosphate buffer saline and sealed for transport for polymerase chain reaction (RT-PCR). The PCR values were obtained as CFU/ml. The values were tabulated, and the statistical analysis was performed. RESULTS: The difference between pre and post use of the mouthwash in group A was statistically significant. In group B, there was statistically no difference between pre and post use of probiotic mouth wash. In addition, clinically comparison of post-test values did not differ either. CONCLUSION: Within the limitations of the study, it can be concluded that the use of probiotic mouthwash may not have clinical benefit in orthodontic patients.
| Item Type: | Thesis (Masters) |
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| Additional Information: | 241719006 |
| Uncontrolled Keywords: | S. Mutans, Stainless steel Brackets, Probiotics, Plaque, Mouthwash, RT-PCR. |
| Subjects: | DENTAL > Orthodontics and Dentofacial Orthopedics |
| Depositing User: | Subramani R |
| Date Deposited: | 24 Feb 2021 02:22 |
| Last Modified: | 24 Feb 2021 02:22 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/14444 |
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