Karvanthan, B (2017) Scientific validation of anti-diabetic, antidyslipidemic and anti-oxidant activities of siddha herbal formulation “Elathy Urundai” in in-vivo and in-vitro models. Masters thesis, Government Siddha Medical College, Chennai.
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Abstract
From the Siddha literature “Sarabenthirar Vaithiya Muraigal” the drug Elathy Urundai was selected for its Anti-diabetic, Anti-dyslipidemic and Antioxidant activities. In this dissertation, Introduction explained about the disease diabetes in Modern aspect, prevalence of Diabetes, mortality and morbidity rate, about the Siddha concept, the disease diabetes in Siddha and role of the drug in treating diabetes. The drug was prepared as per classical Siddha literature, the ingredients were identified and authenticated by Gunapadam experts. In review of literature, many studies were carried out about the botanical aspect and Gunapadam aspect of the drugs. Pharmaceutical review enclosed about the preparation of drug and the pharmacological review established the methodologies. The drug was subjected to various analysis such as physico chemical, biochemical and also instrumental analysis which provided the active ingredients present in the drug. Toxicological study was made according to OECD guidelines showed the study of the drug. Pharmacological studies were done to reveal the Anti-diabetic, Antidyslipidemic activity in animal models and Anti-oxidant activity of Elathy Urundai in DPPH assay. Results and discussion gave justifications to prove the potency of the drug. Conclusion explained the synergistic effect of all active ingredients and activities that supports the study. Thus, the herbal formulation Elathy Urundai which is validated for its safety and efficacy for treating Diabetes mellitus, would be a great drug of choice. CONCLUSION: The drug Elathy Urundai was selected to validate the safety and its efficacy for Diabetes mellitus in animal model (Wistar albino rats). The ingredients of the drug was identified and authenticated by Gunapadam experts. The drug was prepared as per classical Siddha literary procedure and subjected to various studies to reveal the potency and efficacy of the drug. The Organoleptic character and physico chemical studies were made to standardization of the drug Elathy Urundai. From the above studies, the Elathy Urundai was standardized as per AYUSH guidelines. The analysis of biochemical, instrumental was made to know the presence of active ingredients in the drug which is responsible for its activity. Here, the biochemical analysis showed the presence of Potassium, Magnesium, Sodium, Zinc by its synergistic effect, the drug as activity against the disease. In instrumental analysis, FTIR showed the peak values represents the functional groups responsible for its activity. SEM picture explained the particle size of the drug. In ICP-OES described about the absence of heavy metals and its permissible limits which showed the safety of the drug. Toxicity studies revealed about the acute and sub-acute toxicity effect of the Elathy Urundai in rat models. The drug showed no toxicity and mortality in both acute and sub-acute toxicity. According to OECD guidelines, the haematological, biochemical parameters were investigated. There were no significant changes in the functional behaviour and in the normal values. Thus, it was established the safety of the drug administrated when for long time. Pharmacological studies were done on the rat model for Anti diabetic, Anti-Dyslipidemic activity and In vitro study for Anti- oxidant activity. In Diabetes the main pathophysiology is increased oxidative stress which results in the tissue damage and it is the main reason for other complications. Theanti-oxidant property of this drug is mainly due to the presence of phytochemicals (flavonoids, phenols, etc.,) and other active ingredients which involve in scavenging the free radicals and prevents tissue damage and other complications. In Anti diabetic activity, there was significant decreased blood glucose level and slightly increased body weight in the Streptozotocin induced Wistar albino rats. In Diabetes, hyperglycemia is always accompanied with the dyslipidemia. Deficiency of insulin results in the increase in level of LDL, VLDL, TGL, cholesterol and decrease in HDL. Dyslipidemia produces further vascular complications and increases the severity of Diabetes. The drug showed significant decrease in the LDL and marked increase in the HDL than the standard drug. In Anti dyslipidemic activity, showed significant decreased LDL, VLDL, TGL, TC levels and marked increased HDL level in the triton WR-1339 induced dyslipidemic in Wistar albino rat models. In Anti oxidant activity, there was significant effect of drug when compare to standard in DPPH assay. Thus by surviving all the above factors, it is concluded that the drug Elathy Urundai is safe and potent drug with Anti diabetic and Anti-dyslipidemic activity with rich Anti-oxidant activity. This will support the treatment and management of diabetes and its complications. In treating, the disease with this drug it has a synergistic effect of controls blood sugar level, lipid profile and also reduces the oxidative stress and gives a complete treatment for diabetes and its complication as a whole.
| Item Type: | Thesis (Masters) |
|---|---|
| Additional Information: | (Reg.No. 321412107) |
| Uncontrolled Keywords: | Scientific validation ; anti-diabetic ; antidyslipidemic ; anti-oxidant activities ; siddha herbal formulation ; Elathy Urundai ; in-vivo and in-vitro models. |
| Subjects: | AYUSH > Gunapadam |
| Depositing User: | Kambaraman B |
| Date Deposited: | 24 Sep 2018 00:54 |
| Last Modified: | 24 Sep 2018 00:54 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/9595 |
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