Vanathi, S (2015) Isolation, Speciation and Anti Fungal Susceptibility of Dermatophytic Infection in Patients attending a Tertiary Care Hospital. Masters thesis, Kilpauk Medical College, Chennai.
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Abstract
INTRODUCTION: Dermatophytoses are the infection confined to the dead cornified layers such as skin, hair and nail. Dermatophytoses are common in tropical countries like India due to hot and humid climate. Nowadays susceptibility to fungal infection is increasing due to inadvertent use of antibiotics and newer modality of management. Isolation, speciation and antifungal susceptibility pattern aids the clinician to select the appropriate antifungal agent for the management of dermatophytic infection. AIMS AND OBJECTIVES: 1. To isolate the dermatophytes causing infection of skin, hair and nail. 2. To identify and speciate the isolates of dematophytes. 3. To determine the antifungal susceptibility of the isolates. MATERIALS AND METHODS: Specimen collection : Skin, hair, and nail are taken from active edge of the lesion and are examined in 10% KOH to detect fungal hyphae. All specimens are inoculated in duplicate in Modified Sabouraud ‘s Dextrose agar and Dermatophyte test medium which are incubated at 25 - 37°c for 10 days - 2 weeks .Identification was done on the basis of the colony characteristics and microscopic morphology in lactophenol cotton blue mount .The antifungal susceptibility test was done by micro broth dilution method to detect MIC for Griseofulvin, Ketoconazole, Fluconazole and Itraconazole. RESULT: 217 clinically suspected cases of dermatophytes were subjected to mycological study. Most of the cases were seen between 21 – 30 years of age group. Male to Female ratio was 1.58 : 1. 80 (36.86%) samples were showed culture positive. Trichophyton species were the predominant isolates (96.2%) followed by Epidermophyton (2.25%) and Microsporum (1.25%) Trichophyton rubrum was the predominant isolate in Tinea corporis, Tinea cruris, Tinea capitis and Tinea unguium. In vitro hair perforation test and Urease test helps to differentiate Trichophyton mentagrophytes from Trichophyton rubrum. The minimal inhibitory concentration of Griseofulvin was 0.03-0.5μg/ml. The minimal inhibitory concentration of Fluconazole was 1-8 μg/ml. The minimal inhibitory concentration of. Itraconazole was 0.015-0.25 μg/ml. Fluconazole showed a higher MIC value when compared to other drugs by micro broth dilution method. Itraconazole was found to be the most effective of all the drugs tested. CONCLUSION: Totally six species were isolated on culture. Trichophyton rubrum was the predominant isolate followed by Trichophyton mentagrophytes, Trichophyton verrucosam, Trichophyton tonsurans, Epidermophyton flocccosum and Microsporum gypseum DTM are the special medium used for the persumtive diagnosis of dermatophytes. Itraconazole was found to be the most effective of all the drugs tested. Specific identification of the dermatophytic species and timely institution of appropriate antifungal therapy based on the prevailing sensitivity pattern could be of immense value.
| Item Type: | Thesis (Masters) |
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| Uncontrolled Keywords: | Dermatophytoses ; Trichophyton ; Epidermophyton ; Microsporum MIC. |
| Subjects: | MEDICAL > Microbiology |
| Depositing User: | Punitha K |
| Date Deposited: | 23 May 2018 03:11 |
| Last Modified: | 27 May 2018 06:14 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/8034 |
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