Pavithrah, D (2022) Comparison of Nuclear Alterations between the Buccal Mucosa of Tobacco Chewers and Tobacco Smokers using AgNOR and Leishman-Giemsa Cocktail Stain: A Cytological study. Masters thesis, Vivekananda Dental College For Women, Tiruchengode.
Full text not available from this repository.Abstract
INTRODUCTION: Tobacco products contain carcinogenic byproducts that directly or indirectly alter the cells of oral mucosa. Tobacco induced oral mucosal changes can be easily screened cytologically by using special stains like AgNOR (Argyrophilic Nucleolar Organizer Regions) and Leishman – Giemsa (LG) cocktail stains, which are simple chair side procedures that are less time consuming and cost-effective. AIM OF THE STUDY: To compare the nuclear alterations in buccal mucosal cells of tobacco chewers and tobacco smokers using AgNOR and LG cocktail stain. MATERIALS AND METHODS: This study included 90 samples of which 30 were non- tobacco users (controls, Group I), 30 were tobacco chewers (Group II) and 30 were tobacco smokers (Group III). Two oral smears were collected using cytobrush and the nuclear anomalies were analysed after staining one set of smears with AgNOR stain and the other set with LG cocktail stain. Mean AgNOR counts and the nuclear anomalies like Pyknosis (PK), Karyolysis (KL), Karyorrhexis (KR), Condensed Chromatin (CC), Broken Egg nuclei (BE), Micronuclei (MN), Binucleation (BN) and Nuclear budding (NB) were counted. Statistical comparison between the study groups were done using Kruskal Wallis test. RESULTS: The results showed that the mean AgNOR dots were increased in Group III compared to Group II and Group I with a p value of <0.001. The nuclear anomalies studied using LG cocktail stain showed increased total nuclear anomalies in Group II compared to Group III and Group I with a p value of <0.001. And also PK, KL, KR, CC and MN was increased in Group II with a statistically significance difference (p<0.001). BE, BN and NB was not statistically significant between Group II and Group III. CONCLUSIONS: The nuclear alterations in tobacco smokers are more when compared to tobacco chewers using AgNOR. The nuclear alterations like PK, KL, KR, CC and MN using LG stain were more in tobacco chewers than in tobacco smokers. Further studies with large scale population and gender – matched study population can give more precise results.
| Item Type: | Thesis (Masters) |
|---|---|
| Additional Information: | 241921452 |
| Uncontrolled Keywords: | AgNOR, Cytology, LG cocktail stain, Nuclear Alterations, Tobacco. |
| Subjects: | DENTAL > Oral Pathology and Microbiology |
| Depositing User: | Subramani R |
| Date Deposited: | 08 May 2022 08:50 |
| Last Modified: | 14 May 2022 06:45 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/19778 |
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