Joyna Singh, (2022) To Identify and Compare Microbiological Profile of Nasopharynx in Children with Features of Symptomatic Adenoid Enlargement Versus Asymptomatic Normal Children. Masters thesis, Christian Medical College, Vellore.
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Abstract
BACKGROUND: Adenoids, an aggregate of lymphoid tissue situated in the nasopharynx is commonly enlarged in children between the age of two to eight years due to the normal physiological growth pattern. Hypertrophied adenoid can also act as a focus of infection with can lead to ear, sinonasal and repeated respiratory infections. In some children enlargement of adenoids can cause obstructive symptoms including sleep disturbances. The nasopharyngeal microflora consists of pathogenic and commensal bacteria which undergo constant interaction and along with other factors results in destruction of pathogenic bacteria or disease production. Therefore knowledge of the microbiological profile of adenoids in symptomatic and asymptomatic children is relevant. A better understanding of the pathogenic and commensal bacteria and biofilm forming capacity of pathogenic bacteria is the broad theme of this study. AIM OF THE STUDY: The aim is to study the microbiological profile of nasopharynx in children with symptomatic adenoid enlargement and asymptomatic normal children. OBJECTIVES: 1. To compare the microbiological profile of nasopharynx in children below 16 years with symptomatic adenoid enlargement and asymptomatic normal children. 2. To determine the biofilm forming capacity of the organisms in the symptomatic group. METHODOLOGY: The study was a prospective comparative study where 90 children were recruited, 60 in study arm and 30 in control arm . The study group consisted of children with symptoms of adenoid enlargement and control group had asymptomatic children. The history and clinical presentation of each child was documented following which adenoid size was assessed by Rigid nasal endoscopy and or X ray lateral view of nasopharynx. Nasopharyngeal swab was taken after that and porcessed in Department of Microbiology for evaluating nasopharyngeal microflora. Microbiological assessment consisted of detecting the growth of pathogenic or commensal bacteria from the nasopharyngeal swab and semi quantifying it. This was followed by the study to detect the ability of pathogenic bacteria to form biofilm. The strength of biofilm formation and the growth of each pathogenic bacteria on the biofilm was also studied. RESULT: In both study and control group, the predominant microflora was commensal and was present in 70% and 66% of individuals respectively. The most common pathogens in both the group were Staphylococcus aureus and Streptococcus pneumonia. As age progressed, a decreasing trend in the number of commensals and replacement by pathogenic bacteria was observed. Pathogenic bacteria in study and control group had 68% and 55% capacity to form biofilm respectively. Staphylococcus aureus was associated with strong biofilm forming capacity, followed by Streptococcus pneumonia. CONCLUSION: This study compared the microflora of nasopharynx in asymptomatic children and children with adenoid hypertrophy. It helped to gain a better understanding of the changes of microflora in the nasopharynx of a child with advancing age. Early childhood is associated with more commensals in the nasopharynx even in children with adenoid hypertrophy. Biofilm forming capacity of the pathogenic bacteria were also studied.
| Item Type: | Thesis (Masters) |
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| Additional Information: | 221914355 |
| Uncontrolled Keywords: | Identify, Compare Microbiological Profile, Nasopharynx, Children, Symptomatic Adenoid Enlargement, Asymptomatic Normal Children. |
| Subjects: | MEDICAL > Otolaryngology > MEDICAL > Otolaryngology |
| Depositing User: | Subramani R |
| Date Deposited: | 27 Jun 2021 03:10 |
| Last Modified: | 17 Dec 2023 14:47 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/17834 |
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