Deepasri, M (2016) Detection of Epstein Barr Virus in the Whole Mouth Fluid of Oral Cancer, Cervical Cancer Patients and Normal Individuals. Masters thesis, Ragas Dental College and Hospital, Chennai.
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Abstract
BACKGROUND: The first human virus to be directly implicated in carcinogenesis is Epstein Barr Virus. The association of Epstein Barr Virus (EBV) in nasopharyngeal cancer has been established. Its etiological role in oral and cervical cancer remains unclear. AIM AND OBJECTIVES: To quantify the expression of EBV in the whole mouth fluid (saliva) of oral cancer patients, cervical cancer patients and normal individuals by quantitative real time polymerase chain reaction (qPCR). MATERIAL AND METHODS: DNA was extracted from saliva samples of naive oral cancer (n=20), naive cervical cancer (n=10) and normal individuals (n=10). The extracted DNA was subjected to quantitative Polymerase Chain Reaction (q-PCR) to detect and quantify EBV. RESULTS: Among the study group, EBV was isolated in the saliva of 45% (n=9) of oral cancer patients, 40% (n=4) of cervical cancer patients and 50% (n=5) of normal individuals. 66.66% (6/9) of oral cancer patients, 75% (3/4) of cervical cancer patients and 60% (3/5) of normal individuals crossed the threshold level of 1000 copies/ml. The mean EBV viral load was highest in the saliva of cervical cancer patients followed by oral cancer patients and normal individuals. CONCLUSION: EBV was detected in the whole mouth fluid of a subset of oral cancer patients, cervical cancer patients and normal individuals in this present study. Further studies need to be carried out to establish the etiologic role of EBV in the pathogenesis of oral and cervical cancers.
| Item Type: | Thesis (Masters) |
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| Uncontrolled Keywords: | oral cancer, cervical cancer, Epstein Barr Virus (EBV), saliva. |
| Subjects: | DENTAL > Oral Pathology and Microbiology |
| Depositing User: | Subramani R |
| Date Deposited: | 21 Sep 2021 02:14 |
| Last Modified: | 21 Sep 2021 02:14 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/16261 |
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