Karthik, S (2020) Detection and Quantification of Epstein Barr Virus in Saliva and Subgingival Plaque of Paediatric patients with Acute Lymphoblastic Leukaemia. Masters thesis, Ragas Dental College and Hospital, Chennai.
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Abstract
BACKGROUND: Epstein Barr virus (EBV), an oncogenic human herpes virus, has been associated with malignant tumours of epithelial cells and B lymphocytes. Active replication in blood has been reported in 32% of Indian children with ALL by real time PCR. Epstein Barr virus is released into saliva from epithelial cells and saliva is known to play an important role in transmission of Epstein Barr virus. This study was designed to detect, quantify and assess the Epstein Barr virus in saliva and subgingival plaque of paediatric patients with acute lymphoblastic leukaemia and compare with controls. There are reports where EBV had been detected and quantified in saliva in hematopoietic malignancies other than ALL. There are no reported studies that have detected and quantified EBV in saliva and subgingival plaque samples. AIM OF THE STUDY: To study the presence of Epstein Barr virus in saliva and subgingival plaque of paediatric patients with acute lymphoblastic leukaemia. OBJECTIVE: To assess the prevalence of Epstein Barr virus in saliva and subgingival plaque of paediatric patients with acute lymphoblastic leukaemia using quantitative real time polymerase chain reaction. MATERIALS AND METHOD: EBV DNA extraction was done from unstimulated saliva and subgingival plaque samples from healthy paediatric individuals (n=20) and paediatric patients with acute lymphoblastic leukaemia (n=20), as per the protocol of the Indigenous SmartPrepTM Genomic DNA Extraction Kit. The extracted DNA was subjected to quantitative real time Polymerase Chain Reaction (q-rtPCR) to detect and quantify EBV. RESULTS: Statistically significant difference was observed between ALL and controls with respect to age, height, weight, haemoglobin, red blood cell count, white blood cell count, platelet count and mean oral hygiene index. EBV was detected in 4 cases of saliva samples of ALL patients above the threshold value and all these 4 patients also presented with acute respiratory infections. There was a statistical significant difference between the two groups on comparison of threshold values (> 6.841) of final qPCR amplification plot (0.035). Mean EBV viral load in saliva samples in Group I (N=20) was 103812 ± 164452.2040 copies/ml and Group II (N=20) was 88464.0 ± 177418.3829 copies/ml. Statistically significant difference (p=0.004) was observed in the study groups with respect to EBV viral load. CONCLUSION: EBV is present in some ALL patients and should be considered as a factor associated with systemic morbidity. Further studies are needed to ascertain a causal relationship, if any, between EBV and ALL in paediatric patients.
| Item Type: | Thesis (Masters) |
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| Additional Information: | 241721152 |
| Uncontrolled Keywords: | Epstein Barr virus, acute lymphoblastic leukaemia, saliva, subgingival plaque, real time qPCR. |
| Subjects: | DENTAL > Oral Pathology and Microbiology |
| Depositing User: | Subramani R |
| Date Deposited: | 24 Feb 2021 16:21 |
| Last Modified: | 26 Feb 2021 01:03 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/14477 |
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