Levels of pentraxin-3 in gingival crevicular fluid of patients with aggressive and chronic periodontitis: An In Vivo study

Ritika, Chhalani (2018) Levels of pentraxin-3 in gingival crevicular fluid of patients with aggressive and chronic periodontitis: An In Vivo study. Masters thesis, Sri Ramakrishna Dental College and Hospital, Coimbatore.

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Abstract

BACKGROUND: Periodontitis is a multifactorial disease and is a major factor for tooth loss. The pathogenesis of this disease has varied and evolved over the decades. It is now believed that the host has a major role to play in the progression of periodontal destruction. The changes that occur in the periodontium are often hard to explain clinically and require an understanding at the biochemical level. Biomarkers, such as acute phase proteins have the potential to shed more light on the changes occurring within the tissues and are believed to be more reliable. Pentraxin-3 is one such acute phase protein. AIM & OBJECTIVES: The aim of this study was to estimate and compare the levels of Pentraxin-3 in the gingival crevicular fluid of aggressive periodontitis, chronic periodontitis and healthy subjects. MATERIALS & METHODS: A total of 45 subjects were enrolled in this cross-sectional study. The study groups included subjects with chronic periodontitis (n = 15), those with aggressive periodontitis (n = 15) and those who acted as healthy controls (n = 15). Following the assessment of the clinical parameters, sites with the greatest mean sulcular bleeding index score were selected in every subject to serve as a source of gingival crevicular fluid collection. Gingival crevicular fluid was collected using microcapillary pipettes and processed to estimate Pentraxin-3 levels using enzyme linked immunosorbent assay. RESULTS: The values obtained were statistically compared. Overall comparisons were made using one way analysis of variance, pairwise comparisons were made using Tukey HSD and overall correlation was assessed by means of Pearson correlation. The values of Pentraxin-3 were the highest in aggressive periodontitis group followed by chronic periodontitis groups and were the least in the healthy subjects. This overall difference between the groups was found to be of statistical significance. On pairwise comparison, the levels of Pentraxin-3 were higher in the aggressive periodontitis group than in the chronic periodontitis group (mean difference: 4.53ng/mL), in the aggressive periodontitis group than in the healthy group (mean difference: 6.01ng/mL) in the chronic periodontitis group than in the healthy group (mean difference: 1.48ng/mL) with the difference between the groups being statistically significant on each of these comparisons (p < 0.001). Overall, a positive correlation was observed between Pentraxin-3 and full mouth probing depth (r = 0.708), Pentraxin-3 and individual probing depth (r = 0.704), Pentraxin-3 and full mouth clinical attachment level (r = 0.687), Pentraxin-3 and individual clinical attachment level (r = 0.643), Pentraxin-3 and full mouth plaque index (r = 0.495), Pentraxin-3 and mean sulcular bleeding index (r = 0.752) and these correlations were found to be statistically significant in each case (p < 0.001). CONCLUSION: The results obtained from the present study suggest that the levels of Pentraxin-3 rise with increase in activity and severity of periodontal disease. Hence Pentraxin-3 may serve as a valuable biomarker and diagnostic tool in the future.

Item Type: Thesis (Masters)
Uncontrolled Keywords: Pentraxin-3 ; gingival crevicular fluid ; inflammation ; biomarker ; chronic periodontitis ; aggressive periodontitis.
Subjects: DENTAL > Periodontology
Depositing User: Subramani R
Date Deposited: 26 Oct 2018 00:18
Last Modified: 26 Oct 2018 00:18
URI: http://repository-tnmgrmu.ac.in/id/eprint/9962

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