A Comparative evaluation of antibacterial efficacy of neem and turmeric with and without addition of calcium hydroxide against enterococcus faecalis: An In Vitro study

Karthikeyan, A (2018) A Comparative evaluation of antibacterial efficacy of neem and turmeric with and without addition of calcium hydroxide against enterococcus faecalis: An In Vitro study. Masters thesis, Adhiparasakthi Dental College and Hospital, Melmaruvathur.


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Complete disinfect ion of the root canal system is essential for the success of root canal therapy. This requires the use of an intra - canal medicament. Enterococcus faecal is is the most frequently found species in persistent /secondary intracanal infect ion associated endodontic treatment failure. This study evaluates the antibacterial property of neem and turmeric as a intracanal medicament in combination with calcium hydroxide. The activity of neem, turmeric and calcium hydroxide against E. faecal is planktonic cells was measured on well diffusion assay. Broth dilution method was done to determine minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the medicament used. Seven days E.faecal is biofilm was inoculated on root canal surface to check herbal activity with and without addition of calcium hydroxide. Dent in samples were collected on root canal to quantitatively analysing the colony formig units by pour plate’s method. AIM OF THE STUDY: The aim of the invitro study is to compare the antimicrobial activity of neem (Azadirachta Indica)and turmeric (Curcuma Longa) with and with out Addition of Calcium hydroxide Against E.faecal is Biofilm. MATERIALS AND METHODS: Sixty single rooted human mandibular premolars extracted for orthodontic purpose has been selected. The external surface of the teeth and the cementum were cleaned using per iodontal curettes. Samples were decoronated at the level of CEJ to obtain the length of 13mm. Cleaning and shaping were done on rotary protaper universal file up to F3 size. The samples were divided in to 6 Groups (n=10). Group I (neem), Group II(turmeric), Group III (calcium hydroxide), Group IV (neem + calcium hydroxide), Group V (turmeric + calcium hydroxide), Group VI (saline). The tooth samples were inoculated for 7 days in E.faecal is broth. At the end of 7 days tooth samples were rinsed with saline superficially and placed in a medicament for 24 hrs. Dent in samples were collected from the root canal using H files size 30 placed in broth solution. Colony forming units were counted using pourplate’s method. RESULTS: The result of the present study showed that neem + calcium hydroxide combination is a maximum inhibition of bacterial count. Neem alone showed good antibacterial activity when it combined with calcium hydroxide it is showed even improved antibacterial activity compared to all other Groups. Saline (control) showed more colony forming units when compared with other Group. CONCLUSION: Within the limitation of this study i t was found that neem + calcium hydroxide showed maximum antibacterial activity against 7 days E.faecal is biofilm formed on the tooth substrate followed by neem, calcium hydroxide, turmeric + calcium hydroxide. But none were able to completely eradicate E. faecal is biofilm in dentinal tubules. Further research is needed to conclusively recommend herbal as a root canal medicament.

Item Type: Thesis (Masters)
Uncontrolled Keywords: antibacterial efficacy ; neem ; turmeric with and without addition ; calcium hydroxide ; enterococcus faecalis.
Subjects: DENTAL > Conservative Dentistry and Endodontics
Depositing User: Subramani R
Date Deposited: 25 Oct 2018 00:02
Last Modified: 27 Oct 2018 12:31
URI: http://repository-tnmgrmu.ac.in/id/eprint/9930

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