Changes in alkaline phosphatase levels in gingival crevicular fluid and saliva following en-masse retraction: A Comparitive study

Shireen, Cox (2015) Changes in alkaline phosphatase levels in gingival crevicular fluid and saliva following en-masse retraction: A Comparitive study. Masters thesis, Sri Ramakrishna Dental College and Hospital, Coimbatore.


Download (2MB) | Preview

Download (6kB) | Preview


BACKGROUND: Orthodontic tooth movement includes alveolar bone modeling and remodeling processes which can be understood by evaluating the biomarkers of bone metabolism. AIM OF THE STUDY: This study was performed to evaluate the changes in Alkaline Phosphatase levels in Gingival Crevicular Fluid (GCF) and saliva during en-masse retraction with a continuous force of 150 grams and validate the use of saliva as a diagnostic tool in orthodontics. METHODS: Saliva samples and GCF samples from the gingival sulcus in relation to mesio-labial line angle of right and left maxillary canines were collected for 10 patients at baseline, 1 hour, 3 days, 7 days, 14 days and 21 days after force application. The samples were assayed for Alkaline Phosphatase activity spectrophotometrically. The values were calculated statistically by using paired samples t test. RESULTS: There was an increase in enzyme levels from baseline to 21 days. There was no much increase from baseline to 1 hour but a gradual increase was seen till 3rd day followed by a steep increase in 7th and 14th day. There was no much increase on the 21st day. The peak in activity of the enzyme was noted on the 7th day after force application and a sharp decline in the rise of enzyme activity after 14th day. The pattern was found to be the same in GCF and saliva. The growth rates at each time interval was slightly lesser in saliva compared to GCF. CONCLUSION: Alkaline Phosphatase reflects changes in alveolar bone turnover during orthodontic tooth movement. Saliva can be used as an alternative diagnostic tool to GCF to assess Alkaline Phosphatase but it does not provide site-specific changes in alveolar bone. GCF still remains the viable option when accurate, site-specific changes need to be assessed.

Item Type: Thesis (Masters)
Uncontrolled Keywords: Alkaline Phosphatase ; Gingival Crevicular Fluid (GCF) ; saliva ; en-masse retraction ; alveolar bone turnover.
Subjects: DENTAL > Orthodontics and Dentofacial Orthopedics
Depositing User: Subramani R
Date Deposited: 14 Oct 2018 16:58
Last Modified: 14 Oct 2018 16:58

Actions (login required)

View Item View Item