Immunohistochemical evaluation of p-mTOR (Phosphorylated Mammalian Target of Rapamycin) in oral squamous cell carcinoma

Abirami, M (2018) Immunohistochemical evaluation of p-mTOR (Phosphorylated Mammalian Target of Rapamycin) in oral squamous cell carcinoma. Masters thesis, Adhiparasakthi Dental College and Hospital, Melmaruvathur.


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BACKGROUND: Oral cancer is one of the leading causes of cancer death in India. Due to persistence or recurrence of cancer despite aggressive therapy, there is a necessity for the need of treatment modalities that can target the molecules in the signaling pathway that are activated in cancer conditions. The Mammalian Target of Rapamycin (mTOR) is a member of the Phosphatidylinositol 3 (PI - 3)- Kinase- family & downstream molecule in the Akt /mTOR/pS6 signaling pathway which plays a critical role in regulating basic cellular functions including cell proliferation, cell survival, motility and angiogenesis. In carcinoma conditions, there will be dysregulation in the Akt /mTOR/pS6 signaling pathway. Hence, our present study is conducted to find the expression of p-mTOR in different grades of Oral Squamous Cell Carcinoma and to compare it with normal mucosa. AIM OF THE STUDY: The aim of this study is to analyse the Immunohistochemical expression of phosphorylated form of mTOR in different grades of OSCC in comparison with normal mucosa. MATERIALS & METHODS: This study was conducted on the archival retrieved formal in fixed, paraffin embedded tissues obtained from the Department of Oral Pathology, Adhiparasakthi Dental College and Hospital, Melmaruvathur and from private hospitals. The study group included 21 cases of histologically diagnosed Oral Squamous Cell Carcinoma (7 cases of each Well, Moderately and Poorly differentiated squamous cell carcinoma). Control group includes biopsies from the normal buccal mucosa adjacent to the site of surgery during the surgical removal of third molar in 10 patients. 3 micron thickness sect ions were made from each sample and stained with p-mTOR primary antibody. The expression was scored and analysed using Kruskal Wall is test. RESULTS: Statistically significant difference (p=0.03) exists when comparing the intensity of staining of p-mTOR in between different grades of Oral Squamous Cell Carcinoma (OSCC). Increased intensity in the OSCC samples were observed when compared with normal mucosa. This value is also statistically significant (p = 0.003). Also, when comparing the area of staining of p-mTOR in between different grades of OSCC statistically significant difference (p=0.03) exists across these groups. Increased number of positive stained cells were observed in OSCC samples when compared to normal mucosa and the result is statistically significant (p=0.003). CONCLUSION: We analysed the expression of p-mTOR as the first attempt in between different grades of OSCC and compared the expression with normal mucosa. Our present study reveals the significant expression of p-mTOR in Akt /mTOR/pS6 signaling pathway which act as a prognostic marker or target for molecular therapies. Further research with a larger number of sample size with clinicopathologic correlation and long term follow up will emphasize more towards the use of Mtor as a prognostic marker. It will also be more interesting to correlate the presence and frequency of other molecular aberrations such as p-Akt and pS6 to trace out the role of entire Akt/mTOR/pS6 signaling pathway in OSCC. It will also be useful for the development of new therapeutic strategies targeting on mTOR pathway.

Item Type: Thesis (Masters)
Uncontrolled Keywords: p-mTOR ; Akt /mTOR/pS6 signal ing pathway ; Oral Squamous Cel l Carcinoma ; Immunohistochemistry.
Subjects: DENTAL > Oral Pathology and Microbiology
Depositing User: Subramani R
Date Deposited: 14 Oct 2018 08:32
Last Modified: 14 Oct 2018 08:32

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