Expression of survivin in oral potentially malignant disorders: A Retrospective Immunohistochemical study

Rajanna Venkatraman, M (2018) Expression of survivin in oral potentially malignant disorders: A Retrospective Immunohistochemical study. Masters thesis, Best Dental Science College, Madurai.


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AIM OF THE STUDY: To compare the immunohistochemical expression of survivin in oral potentially malignant disorders (OPMDs) and evaluate its prognostic significance in oral leukoplakia (OL), oral submucous fibrosis (OSMF), and oral lichen planus (OLP). MATERIALS AND METHODS: The study material consisted of 60 formalin fixed paraffin embedded tissue samples: 15 cases each of OL, OSMF, OLP, and normal oral mucosal epithelium as control. Survivin expression was analyzed immunohistochemically and data analysis was accomplished using SPSS version 22.0. Fisher’s chi square test was opted to compare the variance in distribution, intensity of staining, percentage of immunopositivity and assess pairwise comparison between the groups (OL and OSMF, OL and OLP, and OSMF and OLP). A 'p' value less than 0.05 denotes significant relationship. RESULTS: Survivin was expressed in all the OPMDs (OL, OSMF and OLP), but absent in normal oral tissue samples. Higher immunoreactivity and survivin staining was observed in OLP compared to OL and OSMF while OL showed a significant difference in the distribution of survivin immunoexpression against OLP. The mean immunopositivity of OSMF was lower than in OLP. An increased nuclear expression of survivin along with distribution in the basal and parabasal layers was evident in all OPMDs. CONCLUSION: Survivin was expressed more in OLP (mostly the erosive type) in comparison to OSMF and OL suggesting an unfavourable prognosis. Also, OL showed increased survivin expression than OSMF thereby indicating greater potential towards malignant transformation. Though higher survivin expression and its nuclear localization appeared to correlate with higher grades of epithelial dysplasia, a positive correlation with malignant transformation could not be established due to the small sample size and retrospective nature of the study. Based on the present study, it was concluded that survivin may be used as an important diagnostic and prognostic marker for OPMDs. To further validate survivin as a prognostic marker, a large scale study with greater sample size along with clinical follow-up data is needed. Due to its selective expression in tumor cells and absence in normal tissues, it may be used as a therapeutic target for oral cancer.

Item Type: Thesis (Masters)
Uncontrolled Keywords: Oral Potentially Malignant Disorders ; Oral Leukoplakia ; Oral Submucous Fibrosis ; Oral Lichen Planus ; Immunohistochemistry ; Survivin ; Prognostic Marker.
Subjects: DENTAL > Oral Pathology and Microbiology
Depositing User: Subramani R
Date Deposited: 14 Oct 2018 06:18
Last Modified: 14 Oct 2018 06:18

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