Analysis of caspase-3 enzyme marker expression in oral squamous cell carcinoma: An Immunohistochemical study

Sharifa, K (2018) Analysis of caspase-3 enzyme marker expression in oral squamous cell carcinoma: An Immunohistochemical study. Masters thesis, Madha Dental College and Hospital, Chennai.


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BACKGROUND: Oral cancer is the most common malignancy of head and neck region its ranking 6th position of all malignancy. The most commonly occuring sites are tongue, buccal mucosa, floor of the mouth, lip and gingiva. Smoking tobacco and pan chewing are the etiological factor for the occurence of oral squamous cell carcinoma. Inhibition or imbalance of apoptosis or programmed cell death leads to cancer. Two pathways are involved in apoptosis 1. Intrinsic or mitochondrial pathway, 2. Extrinsic pathway. Caspase -3 enzymes or involved in the apoptosis. AIM OF THE STUDY: To Study the Immunohistochemical expression of caspase -3 in oral squamous cell carcinoma. MATERIALS AND METHODS: In our present study, totally 25 samples were included to detect immunohistochemical expression of caspase -3. Twenty, formalin fixed, paraffin embedded wax tissues histopathologically diagonsed oral squamous cell carcinoma were included as case (Group I). Five normal buccal and gingival tissue formalin fixed, paraffin embedded wax tissues included as control (Group II). Tissues were sectioned 0.5 micron thickness and stained with caspase-3 antibody. The results were obtained and statistically analyzed by using Man n-Whitney test. RESULTS: Increased expression of caspase -3 was seen in epithelium and connective tissue of oral squamous cell carcinoma tissue. In most of the normal mucosa expression of caspase -3 was absent. The p value for epithelium and connective tissue was p < 0.008 and p < 0.016 respectively which was highly significant. CONCLUSION: The results of our present study demonstrated that increased caspase-3expression was seen in oral squamous cell carcinoma tissues than that of the normal mucosa. These findings indicate the caspase -3 expression response to prognosis of the cancer and this has to be explored in a larger sample for further studies.

Item Type: Thesis (Masters)
Uncontrolled Keywords: Apoptosis ; Caspase-3 ; Oral cancer.
Subjects: DENTAL > Oral Pathology and Microbiology
Depositing User: Subramani R
Date Deposited: 14 Oct 2018 05:58
Last Modified: 14 Oct 2018 05:58

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