Invitro and invivo evaluation of ferulic acid on d-galactosamine induced hepatotoxicity

26063742, - (2008) Invitro and invivo evaluation of ferulic acid on d-galactosamine induced hepatotoxicity. Masters thesis, Vel’s College of Pharmacy, Chennai.

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Abstract

Ferulic acid is a phenolic compound which is a potent antioxidant and posseses various biological activities. D-Galactosamine is a widely used hepatotoxin and the mechanism of liver toxicity induced by D-Galactosamine is due to the free radical generation and oxidative stress. In this study the levels of hepatospecific marker enzyme (AST, ALT, ALP and LDH) increases in isolated rat hepatocytes when intoxicated with GalN. Treatment with ferulic acid effectively restored the levels of marker enzymes and this result suggests that the ferulic acid provides the membrane stabilizing property and thus offer protection to hepatocytes against GalN induced damage. Current study also proved the hepatoprotective activity of ferulic acid against GalN induced liver damage in male wistar rats. In this study GalN intoxication elevated the liver marker enzymes levels in the serum and pretreatment with ferulic acid reduced the leakage of marker enzymes and thus provides the membrane stabilizing property. The hepatic antioxidant enzymes (SOD, CAT, GPx and GR) were found to be diminishing in GalN intoxication and the diminished levels were brought back to near normal by treatment with ferulic acid. It is clearly indicates that the ferulic acid posseses a antioxidant activity through free radical quenching mechanisms. GalN induced free radical attack the lipid present in the cell membrane which may lead to lipid peroxidation effect and produce MDA as byproduct. So MDA is used as an indicator for measuring lipid peroxidation level. In the present study GalN induction shows the elevated level of MDA, pretreatment with FA decrease the extend of lipid peroxidation by scavenging the free radicals. Lipid peroxidation induced by GalN is concurrent with GSH depletion. In the present study diminished level of GSH wear observed in GalN intoxication pre treatment with FA significantly restored the decreased level of GSH to normalcy. In present study, Histophathological examination of toxic control shows the sinusoidal dilatation and inflammation in liver tissue in pretreatment with FA significantly reduced the pathological changes produced by the GalN.

Item Type: Thesis (Masters)
Additional Information: (Reg. No. 26063742)
Uncontrolled Keywords: ferulic acid ; d-galactosamine ; induced hepatotoxicity ; Invitro and invivo evaluation.
Subjects: PHARMACY > Pharmaceutical Biotechnology
Depositing User: Subramani R
Date Deposited: 03 Oct 2018 15:26
Last Modified: 03 Oct 2018 15:26
URI: http://repository-tnmgrmu.ac.in/id/eprint/9651

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