Giftillda Selva Elsee, T (2017) Preclinical evaluation of Analgesic, Anti-inflammatory & Anti-Rheumatic activities of Siddha herbal formulation Keelvayu Nivarana Chooranam in animal model. Masters thesis, Government Siddha Medical College, Chennai.
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Abstract
The trial drug of “Keelvayu Nivarana Chooranam” has been selected and its efficacy was analyzed for the preclinical study to establish the analgesic, acute and chronic anti-inflammatory, anti-rheumatic activity. The drug is easily prepared and preparation is very simple when compared to modern drugs. The trial medicine is cost effective. It contains essential elemental, organic, constituents for its basic and potentiality and it represented the micro particles of its content. The ingredients of the test drug was identified and authenticated by Siddha experts. The drug was prepared as per the procedure and subjected to various studies to reveal its potency and effectiveness against the disease. The phytochemical analysis reveals the presence of alkaloids, tannins, flavonoids, cardiac glycosides, saponins, phenols, proteins and carbohydrates. From the above analysis we came to know the presence of active ingredients responsible for its activity. Biochemical analysis showed the presence of Potassium, Calcium, Magnesium, Sodium and Iron. Thus from these results we come to know the effectiveness of the drug is due to the presence of these constituents and it has a synergistic effect in acting against the disease. The FTIR analysis construe the results that showed the presence of functional groups like alcohols, esters, alkanes, aromatic amines which might be responsible for the presence of anti-rheumatic action of the drug. SEM picture described its morphology and the particle size. Toxicological study of both acute and sub-acute toxicity study were carried out in animal model Wister albino rat according to the OECD guidelines. The toxicity helped to fix the doses as 1/5th and 1/10th for pharmacological activities that were carried out. The treated rats in the toxicity studies did not show any mortality, any untoward clinical sign, any behavioural signs, and alterations in body weight and necropsy findings at the end of the study. This indicates that the dosages administered were below toxic level and proves the safety of the drug. Further acute and repeated 28 day oral toxicity studies were done to evaluate the safety of the drug. The mortality, functional observations, hematological and biochemical investigations were made. There was no significant change seen in the normal values. Thus the toxicological study of the test drug greatly establishes the safety and gives the justification for long time administration. The pharmacological study was carried out in the animal model Wistar albino rats. Three activities were seen in the drug Keelvayu Nivarana Chooranam. The activities were 1. Analgesic activity, 2. Anti-inflammatory activity, 3. Anti-rheumatic activity. Analgesic activity was carried out in Acetic acid induced writhing test the test drug KVNC 100 mg/kg b.wt showed marked analgesic activity. Thus this activity reveals the effect of the drug against Rheumatoid arthritis. Anti-inflammatory of the trial drug Keelvayu Nivarana Chooranam was carried out. This activity reveals the anti-inflammatory property of the test drug. Anti-rheumatic activity was carried out in collagen induced arthritis in Wistar albino rat models. The test drug showed significant decreases inflammation and decreased level of CRP in blood. Thus anti-rheumatic activity of the drug is justified. The anti-rheumatic activity of the drug Keelvayu Nivarana Chooranam is mainly of the presence of the active principles. The analgesic and the anti-inflammatory property of the test drug support the drug in treating RA. In RA is always accompanied with increased level of CRP. The drug showed significant decrease in the level of mentioned the entire above drug compared with than the standard drug. Thus by scrutinizing all the above mentioned factors it is concluded that the test drug Keelvayu Nivarana Chooranam a safe and a potent anti-rheumatic drug. To conclude, the drug Keelvayu Nivarana Chooranam beneficiary values for the therapeutic efficacy for anti-rheumatic activity. It also possesses analgesic, anti-inflammatory activities which support the effective treatment for managing Rheumatoid arthritis.
| Item Type: | Thesis (Masters) |
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| Uncontrolled Keywords: | Preclinical evaluation ; Analgesic ; Anti-inflammatory ; Anti-Rheumatic activities ; Siddha herbal formulation ; Keelvayu Nivarana Chooranam ; animal model. |
| Subjects: | AYUSH > Gunapadam |
| Depositing User: | Subramani R |
| Date Deposited: | 02 Oct 2018 13:40 |
| Last Modified: | 02 Oct 2018 13:40 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/9637 |
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