Deepa, G (2017) Scientific Validation of Potent Anti-cervical cancer, Anti- tumor and Anti-oxidant activities of Siddha Herbo Mineral Formulation Rasa Parpam in in-vitro studies. Diploma thesis, Government Siddha Medical College, Chennai.
|
Text
320201517deepa.pdf Download (10MB) | Preview |
Abstract
Trial drug RASA PARPAM was selected from the classical Tamil literature “Aathma Raksha Mirtham Ennum Vaithiya Saara Sangiragam” written by Kandhasamy Mudhaliyar forits anti-cancer activity. The dissertation was started with an introduction explaining about the Siddha concept, prevalence of cervical cancer and role of the test drug in treating cancer cervix. Review of literature in various categories was carried out. It was elaborated under Gunapadam and Modern aspect of ingredients, Siddha and modern aspect of that disease, pharmaceutical aspect and pharmacological aspect in both Siddha and modern. All the ingredients were identified and authenticated by experts. The compound was prepared properly by given procedure in an appropriate situation. The end product underwent standardization parameters in Siddha. The drug was subjected to analysis such as physiochemical, biochemical and instrumental which provided the key ingredients present in the drug thus it accounts forthe efficacy of the drug. The sample was also analysed for anti-microbial load to ensure its accuracy. For the study protocol, required animals were approved by the IAEC under CPCSEA. Toxicological study was made according to OECD guidelines comprising both acute and repeated oral dose 28days toxicity studies in wistar albino rats. It showed the safety of the drug which attributes its utility for long time administration. Pharmacological studies were completed. It proved the Anti-cancer activity through HeLa cell line, anti-tumor activity through HeLa and SiHa cell line models and anti-oxidant activity by DPPH assay of RASA PARPAM. Results and discussion gives the essential validations to prove the potency of the drug. Conclusion gives a compiled form of the study and explains the synergistic effect of all the key ingredients and activities that supports the study. CONCLUSION: Cervical cancer is the second most common malignancy found among women worldwide and some are highly resistant to radiotherapy. The other chemotherapy drugs causses intolerable side effects which are worse than the disease. This paved way for a novel anticancer drug which cures cervical cancer in a non invasive method. The intention of this study is to provide a solution for the above need for a non violent anticancer drug to cervical cancer. Rasa Parpam was chosen as a trial drug from the Siddha literature “Aathma Raksha Mirtham Ennum Vaithiya Saara Sangiragam” written by Kandhasamy Mudhaliyar which was categorized by the department of AYUSH as a classical text. Throughout the study, the safety and efficacy were tested thoroughly. The procedure for drug preparation and its techniques for standardization revealed GMP. The trial drug RSP has satisfied all parameters of testing protocol for Parpam which was assigned by AYUSH. It showed the accurate production and potency of Rasaparpam. Physico-chemical analysis revealed better bio-availability and richness of its mineral content. Favouring this study were the presence of inorganic matters which were found through experiments for analyzing acid and basic radicals. Various instrumental analysis of Rasaparpam such as FT-IR spectroscopy, Raman spectroscopy and scanning electron microscope demonstrated its chemical constituents, functional groups and particle size to support its indication to counter cervical cancer. The anti-microbial activity of trial drug was also considered for its potential Under OECD guidelines, the acute and 28 days repeated oral toxicity studies proved the safety of Rasaparpam at particular dose level. It is very useful in therapeutic dose determination. The pharmacological activities are justified by anticancer effect on HeLa cell lines, anti-tumour effect on HeLa and SIHA cell lines and quantitative measurement of antioxidants by DPPH assay. Factors like safety, efficacy, long self like, bio-availability, presence of significant elements, anions and cations and minerals favouring the activity justifies the main perspective of this study. Anti-cancer effect could be validated scientifically. Due to its Non-toxic anticancer effect, it would benefit the female community in the world.
| Item Type: | Thesis (Diploma) |
|---|---|
| Additional Information: | (Reg. No: 321412101) |
| Uncontrolled Keywords: | Scientific Validation ; Potent Anti-cervical cancer ; Anti- tumor ; Anti-oxidant activities ; Siddha Herbo Mineral Formulation ; Rasa Parpam ; in-vitro studies. |
| Subjects: | AYUSH > Gunapadam |
| Depositing User: | Subramani R |
| Date Deposited: | 02 Oct 2018 13:40 |
| Last Modified: | 02 Oct 2018 13:40 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/9635 |
Actions (login required)
 |
View Item |
