P16INK4a /KI-67 immunocytochemistry in improving the predictive value for high grade cervical intraepithelial (≥ CIN2) lesions in pap smear

Vinoth Kumar, G (2018) P16INK4a /KI-67 immunocytochemistry in improving the predictive value for high grade cervical intraepithelial (≥ CIN2) lesions in pap smear. Masters thesis, Christian Medical College, Vellore.

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Abstract

BACKGROUND: Cervical cytology has limited sensitivity to detect cervical precancerous lesion. Human papilloma virus high risk (hr-HPV) DNA testing is highly sensitive but specificity is limited. We assessed whether p16INK4a/ki-67 dual stained cytology can improve the predictive value for high grade cervical (CIN2+) lesions. AIM/OBJECTIVE: To assess the significance of P16/Ki-67 immunocytochemistry in improving the predictive value for high grade cervical intraepithelial (≥ CIN 2+) lesions on Pap smear. MATERIAL AND METHODS: This is a prospective diagnostic study which included 94 cases of ASC-S/LSIL/ ASC-H and HSIL on thin prep cervical smears who also underwent-HPV DNA test and colposcopy guided biopsy between July 2016 to March 2017. P16INK4a/Ki-67 immunocytochemistry was performed in the residual thin prep samples. Sample for hr-HPV DNA test byHybrid Capture 2 assay were collected during colposcopy. Biopsy is the gold standard against which performance of P16INK4a /Ki-67 and hr-HPV results were compared. RESULTS: In women of all ages, sensitivity (96.8%) and negative predictive value (97.6%) for hr-HPV test and p16/Ki-67 dual immunocytochemistry (≥ 1 positive cell) were similar, but the latter test showed better specificity (70.2 vs 55.8) and positive predictive value (61.2 vs 51.7) for ≥ CIN 2 lesions. A higher cut off of at least 10 positive cells gives a clinically and statistically higher specificity and positive predictive value, while however, slightly decreased sensitivity and negative predictive value. CONCLUSION: Since High risk HPV test has high sensitivity and negative predictive value while P16/Ki-67 dual immunocytochemistry (≥ 10 positive cells) has high specificity and positive predictive value, the latter can be recommended as an ancillary test in hr-HPV test positive women, to reduce the number of women going for colposcopy and biopsies.

Item Type: Thesis (Masters)
Additional Information: Reg.No: 201513357
Uncontrolled Keywords: ASC-US ; LSIL ; ASC-H ; HSIL ; P16INK4a/ki-67 ; hr-HPV DNA ; Cervical Intraepithelial Neoplasia – CIN 2+.
Subjects: MEDICAL > Pathology
Depositing User: Subramani R
Date Deposited: 19 Aug 2018 06:42
Last Modified: 19 Aug 2018 06:42
URI: http://repository-tnmgrmu.ac.in/id/eprint/9318

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