Role of CD10 expression in colorectal carcinoma and premalignant lesions

Abinaya, D (2018) Role of CD10 expression in colorectal carcinoma and premalignant lesions. Masters thesis, Stanley Medical College, Chennai.

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Abstract

INTRODUCTION: Continuous and bilateral molecular interaction between normal epithelial cells and stromal cells is disrupted by several factors secreted by tumour cells or by stromal cells under the influence of tumour cells. CD 10 is a matrix metalopeptidases involved in carcinogenesis through the release of bioactive molecules that stimulate invasion, extracellular matrix degradation, inhibition of apoptosis and promotion of angiogenesis and immune response modulation. OBJECTIVE: 1. To study the immunohistochemical expression of CD10 in colorectal carcinoma and in premalignant lesion. 2. To correlate the expression of CD10 in tumour cells and stromal cells with various clinico pathologic variables. MATERIAL AND METHODS: The study was conducted during the period of August 2016 to August 2017. It was carried out in specimens obtained from patients with confirmed histopathological diagnosis of colorectal adenomas and adenocarcinomas. The study was approved by the Ethical committee of Government Stanley Medical College and hospital. Study Design: Retrospective and comparative study. Study Population: CASES: The study sample comprised of 60 colorectal adenoma and adenocarcinoma patients. Cases were chosen from the Department of Surgical Gastroenterology, Department of Surgery, Government Stanley Medical College and hospital. Age, sex, tumor site, histological grade and tumor stage were obtained for all cases. All the 60 cases were screened for CD 10 expression through immunohistochemical assay. Inclusion Criteria: Patients with colorectal adenomas and adenocarcinomas. Exclusion Criteria: 1. Chemotherapy and/or radiotherapy prior to sampling. 2. Recurrent and metastatic adenocarcinomas. 3. Cancer types other than adenocarcinomas. METHOD OF DATA COLLECTION: A total of 60 cases, 30 each of colorectal adenoma and colorectal adenocarcinoma were studied at random and selected applying the inclusion and exclusion criteria. The tissues so obtained were processed and sectionswerecutat5microns.Hematoxylinand eosin stained sections were stained and analysed. The clinico-pathological characteristics including age, sex, tumor site, histological diagnosis (Adenoma/adenocarcinoma), histological grade (adenomas- low grade/high grade dysplasia ; adenocarcinoma – low/high) and adenocarcinoma stage were obtained for all the cases. Method of Tissue Preparation: 10% neutral buffered formalin was used for fixing the specimens. The tissues were processed in various grades of alcohol and xylene. Paraffin blocks were prepared and sections of 5 micron thickness were cut in semiautomatic microtome. Staining was done by haematoxylin and eosin. Suitable blocks were chosen for immunohistochemistry. CONCLUSION: The aim of the present study was to examine the expression of CD10, cell membrane metallopeptidase in colorectal neoplasia, its role in the transition sequence from adenoma to adenocarcinoma and its association with various clinicopathological characters of adenocarcinomas. The following are the conclusions of the present study. • CD 10 expression showed a significant increase from adenoma to adenocarcinoma. This signifies that CD10 plays an important role in all stages of the adenoma-carcinoma sequence, which includes the early event of adenoma formation from normal epithelium and its malignant transformation. • The expression of CD10 showed significant correlation with Depth of tumour invasion and nodal status. • These results highlight the association of CD 10expression with malignant behaviour of colorectal adenocarcinomas and CD10 could prove to be a new biomarker for aggressiveness and prognostic information in these tumors. • There was however no correlation between CD 10expression and the age and tumour stage. • The finding of gradually increasing CD 10 expression in adenoma to significantly higher expression in adenocarcinomas makes CD 10 an attractive and potential therapeutic target, which when implemented will result in maximal targeting of cancerous and also pre cancerous tissues with minimal damage to the surrounding normal mucosa.

Item Type: Thesis (Masters)
Uncontrolled Keywords: Cluster of differentiation 10 (CD10) ; colorectal carcinoma ; premalignant lesions.
Subjects: MEDICAL > Pathology
Depositing User: Subramani R
Date Deposited: 19 Aug 2018 02:23
Last Modified: 19 Aug 2018 02:23
URI: http://repository-tnmgrmu.ac.in/id/eprint/9296

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