A comparative study on ziehl neelsen staining and immuno histochemical analysis in suspected tuberculous lesions

Veera Raghavan, G (2018) A comparative study on ziehl neelsen staining and immuno histochemical analysis in suspected tuberculous lesions. Masters thesis, Madras Medical College, Chennai.


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BACKGROUND: The diagnosis of extra pulmonary tuberculosis in tissue sections has always been challenging because of the low yield of bacilli in formalin fixed, paraffin embedded tissues. AIM OF THE STUDY: The aim of the present study is to evaluate the diagnostic value of immunohistochemical analysis using rabbit polyclonal mycobacterium tuberculosis antibody and compare the immunohistochemical analysis with Ziehl-Neelsen (ZN) staining in tissue sections of suspected tuberculous lesions. MATERIALS & METHODS: The study sample was 50 suspected cases of extra pulmonary tuberculosis. Tissue sections from the cases were subjected to hematoxylin and eosin staining, Ziehl-Neelsen staining and Immunohistochemical staining using polyclonal antibody to Mycobacterium tuberculosis followed by a comparative analysis of the results. Mycobacterium tuberculosis infected lung tissue which were positive for AFB by Ziehl-Neelsen staining was used as positive control. RESULTS: 12% of cases were positive for acid fast bacilli by Ziehl-Neelsen staining, whereas 82% of cases were positive by immunohistochemical staining. The P value was found to be less than 0.001 by McNemar test which is statistically significant which shows that immunohistochemistry is better than Ziehl-Neelsen staining for localization of the tubercle bacilli. CONCLUSION: Immunohistochemistry can be a reasonable if not better alternative to Ziehl-Neelsen staining or as an adjunct in the diagnosis of extra pulmonary tuberculosis. It is a simple, robust, cheap and sensitive test that can be employed in routine pathology laboratories where results can be available in a day, prompting early diagnosis and treatment.

Item Type: Thesis (Masters)
Uncontrolled Keywords: KEYWORDS: Extra pulmonary tuberculosis ; Mycobacterium tuberculosis ; Granulomatous inflammation ; Immunohistochemistry ; Ziehl-Neelsen staining.
Subjects: MEDICAL > Pathology
Depositing User: Subramani R
Date Deposited: 19 Aug 2018 01:30
Last Modified: 19 Aug 2018 01:30
URI: http://repository-tnmgrmu.ac.in/id/eprint/9289

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