Sharanya, R (2018) Identification, characterisation and antimicrobial resistance pattern of non fermenting gram negative bacilli from various clinical isolates. Masters thesis, Kilpauk Medical College, Chennai.
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Abstract
INTRODUCTION: The Nonfermentative gram-negative bacilli are a group of aerobic, non-sporing bacilli that do not either use carbohydrates as a source of energy or degrade them through metabolic pathways other than fermentation. Non-fermentative gram negative bacilli account for ≥ 15% of isolates from most clinical specimens1 Hospital acquired infections in the acute care units are major threat to patient safety. AIM OF THE STUDY: To identify, characterise and detect antimicrobial resistance pattern of non fermenting gram negative bacilli from various clinical isolates. OBJECTIVES: 1. To isolate and speciate the nonfermenting Gram negative bacilli. 2. To characterise the non-fermenting Gram negative bacilli isolated. 3. To find out the antimicrobial resistance pattern of the non-fermenting Gram negative bacilli isolated. 4. To detect the production of extended spectrum of betalactamases and metallobetalactamases (MBL). 5. To identify the genes responsible for acquired MBL production MATERIALS AND METHODS: The study was conducted in the Department of Microbiology, Kilpauk Medical College and Hospital, Chennai from January 2015 to January2016. Nonfermenting gram negative bacilli were identified using standard identification methods. The antibiotic susceptibility testing was done by Kirby-Bauer disc diffusion method and interpreted according to CLSI guidelines, 2016. MIC for Imipenemwere determined by E Test (MIC strip method). The specific Imipenem-resistant genotype Bla IMP and Bla VIM were determined by polymerase chain reaction (PCR). RESULT: In our study, 200 nonfermenting gram negative bacilliwere isolated from various clinical samples.Maximum samples were obtained from pus samples especially from Burns ward and the maximum risk factors associated are Diabetes Mellitus. Most predominant organism is Pseudomonas aeruginosa followed by Acinetobacter baumannii. Pseudomonas aeruginosa and Acinetobacter baumannii shows higher sensitivity to Pipercillin tazobactem and Imipenem. In Pseudomonas aeruginosa, Acinetobacter baumannii ESBL producers, MBL producers and AmpC producers were seen. MBL-positive isolates were confirmed to be positive for the gene BlaVIM and no Bla IMP. CONCLUSION: The present study showed emerging resistance of non fermenting gram negative bacilli,that can be controlled by strictenforcement of antibiotic policy along with strict infection control measures to prevent further emergence and spread of antibiotic resistance.
| Item Type: | Thesis (Masters) |
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| Uncontrolled Keywords: | Non fermenting gram negative bacilli ; Antibiotic susceptibility pattern ; Imipenem Resistant Pseudomonas. |
| Subjects: | MEDICAL > Microbiology |
| Depositing User: | Subramani R |
| Date Deposited: | 12 Jul 2018 17:51 |
| Last Modified: | 12 Jul 2018 17:51 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/8717 |
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