Collagen Membrane Degradation delayed by Tetracycline and Its Semi-Synthetic Analogue Doxycycline - An In Vitro Study.

Gaurav, Arora (2013) Collagen Membrane Degradation delayed by Tetracycline and Its Semi-Synthetic Analogue Doxycycline - An In Vitro Study. Masters thesis, Tamilnadu Government Dental College and Hospital, Chennai.

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Abstract

BACKGROUND: Successful periodontal regeneration relies mainly on the re-formation of an epithelial seal, deposition of new acellular extrinsic fiber cementum and insertion of functionally oriented connective tissue fibers into the root surface, and restoration of alveolar bone height. The use of collagen membranes for treatment of periodontal defects in regenerative procedures are well known. Collagenase are enzymes responsible for degradation of collagen membranes which is detrimental to the success of periodontal regeneration. It has been well proven that tetracycline and doxycyline have role in inhibition of collagenase enzyme. AIM OF THE STUDY: To evaluate the effects of varying concentration of tetracycline and doxycycline on the rate of degradation of bioresorbable collagen membranes by clostridial collagenase when immersed in vitro. MATERIALS AND METHODS: The rate of collagen membrane degradation were analyzed quantitatively and qualitatively using spectrophotometric and microscopic analysis respectively in vitro when collagen membranes were incubated in PBS alone (group A), Tetracycline hydrochloride at varying concentration with PBS (group B) and Doxycycline hyclate at varying concentration with PBS (group C) observed over day 2, 4, 7 and 14 respectively . Groups were further divided into group I (treated with collagenase) and group II (treated without collagenase). Free protein content in the medium of each sample were analyzed using spectrophotometric analysis. Each collagen membrane sample was observed microscopically to get the overview of general surface topography and organization of collagen fibrils after incubation over time. RESULTS: There was a statistically significant difference in free protein release in the medium over the time between groups with and without collagenase and also there was a statistically significant difference between group with tetracycline and group without any drug and between group with doxycyline and group without any drug and between group with tetracycline and doxycycline in chlostridial collagenase. CONCLUSION Tetracycline at 50 mg/ml concentration and doxycycline at 20 mg/ml concentration are effective in delaying collagen membrane degradation in clostridial collagenase in vitro. Also doxycyline 20 mg/ml is more effective in prolonging the collagen membrane degradation time when compared to tetracycline 50mg/ml concentration.

Item Type: Thesis (Masters)
Uncontrolled Keywords: Tetracycline hydrochloride ; doxycycline hyclate ; collagen membrane Degradation ; collagenase.
Subjects: DENTAL > Periodontology
Depositing User: Subramani R
Date Deposited: 03 Jul 2017 15:39
Last Modified: 04 Jul 2017 06:35
URI: http://repository-tnmgrmu.ac.in/id/eprint/857

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