Role of Ki-67 and p16 as markers of prognostic indices in premalignant and malignant lesions of cervix

Dina Mary, M (2016) Role of Ki-67 and p16 as markers of prognostic indices in premalignant and malignant lesions of cervix. Masters thesis, Tirunelveli Medical College, Tirunelveli.

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Abstract

INTRODUCTION: Since cervical cancer is highly attributed to the effects of HPV, an infectious agent, it can either be prevented or treated at a pre-invasive stage there by reducing morbidity and mortality ; hence the need for additional biomarkers and parameters of cell proliferation and cell death as important diagnostic and prognostic tools. This study was done to evaluate the proliferative indices in cervical pre cancerous and cancerous lesions i.e, mitotic count in hematoxylin and eosin sained sections and Ki-67 and p16 in IHC stained sections. AIMS AND OBJECTIVES: 1. To collect data on occurrence of cervical pre malignant and malignant lesions reported at TVMCH. 2. To evaluate the proliferative indices in cervical pre-cancerous and cancerous lesions - mitotic count in haematoxylin and eosin sections and Ki-67 and p16 in IHC stained sections. 3. To correlate the proliferative indices with varying grades of pre malignant lesions and various histological sub types of cervical carcinoma. MATERIALS AND METHODS: A total of 50 cases including CIN-1,CIN-2,CIN-3 and malignancy of the cervix were evaluated for mitotic figure count using light microscopy on H&E stained sections. Sections devoid of artefact were selected to count 1000 cells for mitotic figures and it was expressed in percentage. Strong nuclear positivity for Ki-67 was considered positive. The scoring was graded 1, 2, 3 for 10-30%,30-50% and > 50%. Staining intensity for p16 (nuclear or/and cytoplasmic staining) was done calculating the number of positive cells. The results were categorised as Grade 1,2,3 for 1-10%,10-50% and >50%positive cells. CONCLUSION: Ki-67 and p16 expression were seen in CIN-I, II, III and squamous cell carcinomas of the cervix.Ki-67 expression was in increasing grades from CIN to carcinoma. p16 had a better expression in high grade intra epithelial lesions (60-80%) and malignancies (100%)of the cervix compared to low grade dysplasias (21%). The mean mitotic count also showed an increase with increasing dysplasia. Thus Ki-67 and p16 expression in pre malignant and malignant lesions of the cervix can be used in conjunction with the histo morphological features to study their proliferative potential.

Item Type: Thesis (Masters)
Uncontrolled Keywords: Ki-67, p16, CIN, Carcinoma cervix.
Subjects: MEDICAL > Pathology
Depositing User: Punitha K
Date Deposited: 13 Jun 2018 02:40
Last Modified: 13 Jun 2018 02:40
URI: http://repository-tnmgrmu.ac.in/id/eprint/8448

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