Sankar Ganesh, T (2016) Immunohistochemical analysis of P53 expression in oral cavity lesions and its relation with poor personal history. Masters thesis, Tirunelveli Medical College, Tirunelveli.
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Abstract
Oncoprotein p53 actively participates in oral carcinogenesis, in its initiation and/or promotion of the process. The expression of mutant p53 has been shown to be present in many human solid tumours of squamous origin including oral squamous cell carcinomas. The wild-type p53 acts as a tumour suppressor recessive gene, the inactivation of which could lead to malignant transformation. The wild-type p53 protein appears to block the cell cycle at the GUS boundary of the cell cycle and to produce GI arrest which provides sufficient time for DNA repair. On the other hand, its mutant variety forms complexes with the wild-type, inactivates the wild-type, and thus prevents the wild-type from carrying out its normal function. This may, in turn, lead to abnormalities in the cell cycle repair system andeventually to malignant transformation. Mutations in p53 gene may provide the cells with a growth advantage over the normal cells leading to malignant transformation. This study investigated the expression of oncoprotein p53 in oral cavity lesions s (60 samples) from tertiary care hospital Tirunelveli medical college – Tirunelveli, using monoclonal antibodies with the polymer-HRP (Horseradish peroxidase) indirect immunohistochemical staining methods. 46/60 Cases were positive for anti-p53 antibody staining and most of the positive specimens showed diffuse pattern of staining, where the cells were seen as small islands. Statistical analysis shows that there is a significant correlation between the expression of p53, cigarette/beedi smoking and the tobacco chewing habits.As the mutant oncoprotein p53 seems to play a role in malignant transformation, more cells are likely to show a strong nuclear staining reaction when compared to normal or hyperplastic oral epithelia. The significance of p53 oncoprotein in head and neck tumourigenesis is yet to be understood. Further studies such as Direct Nucleotide Sequencing, Polymerase Chain Reaction (PCR) and Single Strand Conformation Polymorphism (SSCP), addressing the possible involvement of this oncoprotein are warranted to get an insight into the molecular details of the mechanism.
| Item Type: | Thesis (Masters) |
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| Uncontrolled Keywords: | Oral squamous cell carcinoma ; p53 oncoprotein ; smoking ; tobacco chewing. |
| Subjects: | MEDICAL > Pathology |
| Depositing User: | Punitha K |
| Date Deposited: | 13 Jun 2018 01:38 |
| Last Modified: | 13 Jun 2018 01:38 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/8445 |
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