Vidhya, V R (2015) Detection of NS1 Antigen/IgM, IgG Antibodies in Early Dengue Virus Infection among the Patients attending Sree Mookambika Institute of Medical Sciences, Kulasekharam. Masters thesis, Sree Mookambika Institute of Medical Sciences, Kulasekharam.
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Abstract
INTRODUCTION: Dengue has become a major international public health problem due to human morbidity and mortality it causes. The disease presents as acute febrile illness with chills, headache, retrooular pain, body aches and arthralgia in more than 90% of apparent cases accompanied by nausea, vomiting and a maculopapular rash resembling measles. Aedes aegyptyi is the primary vector mosquito. Dengue virus is a positive stranded encapsulated, RNA virus. Dengue NS1 antigen has been detected in the serum of DEN virus infected patients as early as one day of onset of symptoms. In this study the potential use of dengue NS1 antigen and IgM and IgG antibodies in early dengue diagnosis has been analysed. MATERIALS AND METHODS: The study group consists of all febrile patients with fever of duration less than 5 days attending the medical OPD for a period of one year from July 2013. A total of 176 blood samples from fever patients were received in the Microbiology lab in our instituition. Each sample was subjected to an immunochromatographic test (card test) for NS1 antigen, IgM and IgG antibodies. This was followed by ELISA for NS1 antigen, IgM and IgG antibodies. The other parameters such as age, duration of fever, gender and platelet count were also recorded. RESULTS: Out of 176 samples tested, 57 (32%) were positive only for NS1 antigen, 7 (4%) samples were found to be positive for IgM antibodies and none was found to be positive for IgG antibodies by immunochromatographic test . The 57 samples which were found to be positive by NS1 ELISA were also found to be positive by immunochromatographic test. By ELISA method 37 samples (21%) were positive for IgM and 7 samples (4%) were found to be positive for IgG. Out 57 cases positive for NS1 antigen, thrombocytopenia was evident in 42 cases (74%). In antibody positive cases thrombocytopenia was noted in 20 out of 44 cases (45%). CONCLUSION: NS1 antigen detection is a useful tool in the early diagnosis of dengue infection and deserves inclusion in the diagnostic algorithm for suspected dengue fever cases. The results of NS1 antigen ELISA is comparable to that of NS1 antigen ELISA .But in case of antibodies the immunochromatographic test is inferior to that of ELISA.
| Item Type: | Thesis (Masters) |
|---|---|
| Uncontrolled Keywords: | Dengue ; NS1 antigen ; IgM & IgG antibodies ; platelet ; immunochromatographic test ; ELISA ; Early detection of dengue virus. |
| Subjects: | MEDICAL > Microbiology |
| Depositing User: | Punitha K |
| Date Deposited: | 23 May 2018 03:37 |
| Last Modified: | 27 May 2018 08:08 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/8052 |
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