Detection and Characterisation of Metallo Beta Lactamase Production in Pseudomonas Aeruginosa by Phenotypic and Molecular Methods from Clinical Isolates in a Tertiary Care Hospital

Sri Devi, V G (2015) Detection and Characterisation of Metallo Beta Lactamase Production in Pseudomonas Aeruginosa by Phenotypic and Molecular Methods from Clinical Isolates in a Tertiary Care Hospital. Masters thesis, Tirunelveli Medical College, Tirunelveli.

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Abstract

INTRODUCTION: P.aeruginosa is an opportunistic pathogen associated with a range of nosocomial infections. It flourishes as a saprophyte, with innate resistance to many antibiotics. In addition to its innate resistance, acquired resistance is particularly associated with indiscriminate use of antimicrobials. Carbapenams is the last resort drug used against this organism isolated from patients. Resistance to carbapenams has emerged by various mechanisms; one growing factor leading to the resistance is the production of metallo beta lactamases. With worldwide increase in the occurrence and dissemination of MBLs, early detection is crucial, the benefits of which include timely implementation of strict infection control practices and treatment with alternative antimicrobials. OBJECTIVE: 1. To detect the prevalence of MBL production in Pseudomonas aeruginosa isolates in Tirunelveli Medical College. 2. To detect the MBL production in Pseudomonas aeruginosa isolates by phenotypic and genotypic methods. 3. To evaluate various phenotypic methods in the detection of MBL. METHODS AND MATERIALS: This study will be conducted in the Department of Microbiology in Tirunelveli medical college hospital after approval from the institutional ethical committee. A total of 100 consecutive non repetitive clinical isolates of P.aeruginosa are subjected to three different phenotypic methods such as combined disc test (CDT), double disc synergy test (DDST), MBL E test using Meropenem and confirmed genotypically by RT-PCR for the presence of bla IMP and bla VIM gene. RESULTS: Out of 100 P.aeruginosa isolates, 21 were resistant to Meropenem. Out of 21 Meropenem resistant P.aeruginosa isolates 14 were detected as MBL producer by CDT, 12 and 10 were detected as MBL producer by DDST and MBL E test respectively. The PCR detected 10 isolates as MBL producer and all of them were positive for bla VIM gene and no bla IMP gene was detected in any of the isolates. CONCLUSION: This study shows that DDST is the simple and cost effective method in the detection of MBL and it should be confirmed with the MBL E test if PCR is not available in the clinical laboratory settings.

Item Type: Thesis (Masters)
Uncontrolled Keywords: P.aeruginosa ; Meropenem ; MBL ; Phenotypic methods ; PCR.
Subjects: MEDICAL > Microbiology
Depositing User: Punitha K
Date Deposited: 23 May 2018 03:30
Last Modified: 27 May 2018 06:46
URI: http://repository-tnmgrmu.ac.in/id/eprint/8043

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