Vijayashree, V (2015) Isolation and Charecterization of Uropathogens with Conventional and Chromogenic Media and Detection of Extended Spectrum Beta Lactamases. Masters thesis, Coimbatore Medical College, Coimbatore.
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Abstract
BACKGROUND: Urinary tract infections are one of the most common bacterial infections in humans. It is also one of the common specimens sent to the laboratory. Worldwide, the data show that there is an increasing resistance among the organisms which cause UTI. A study on the changing antibiotic resistance pattern is pertinent for an appropriate treatment and for the prevention and control of the different mechanisms of resistance. AIM OF THE STUDY: To compare the efficacy of chromogenic media with conventional media for the isolation and identification of uropathogens and also to detect their antibiotic susceptibility pattern. To detect the extended spectrum betalactamases production among Escherichia coli, Klebsiella pneumoniae and Proteus by double disc synergy test and Etest. METHODS: A total of 422 samples collected from various departments during the period of August 2013- September 2014 were processed in blood agar, MacConkey agar and chromogenic agar. Isolation rate was compared and antibiotic susceptibility tests were done. ESBL isolates were detected using DDST and E test. RESULTS: Escherichia coli was the common isolate. Chromagar detected all the uropathogens. Escherichia coli was the most common ESBL producer (67.5%). E test was more sensitive than DDST in detecting ESBL. CONCLUSION: ESBL isolates are more susceptible to Meropenem, Nitrofurantoin, and Amikacin. DDST can also be adopted as a method of ESBL detection.
| Item Type: | Thesis (Masters) |
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| Uncontrolled Keywords: | Urinary tract Infections (UTI) ; CHROM AGAR ; Extended Spectrum Beta Lactamases (ESBL) ; AST. |
| Subjects: | MEDICAL > Microbiology |
| Depositing User: | Punitha K |
| Date Deposited: | 23 May 2018 03:25 |
| Last Modified: | 27 May 2018 06:36 |
| URI: | http://repository-tnmgrmu.ac.in/id/eprint/8040 |
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