Study on the Prevalence of Sexually Transmitted Infections with Special Preference to Genital Herpes Infection in the High Risk Groups Attending STD Clinic in a Tertiary Care Hospital

Arulselvan, S (2015) Study on the Prevalence of Sexually Transmitted Infections with Special Preference to Genital Herpes Infection in the High Risk Groups Attending STD Clinic in a Tertiary Care Hospital. Masters thesis, Stanley Medical College, Chennai.


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INTRODUCTION: High risk group of people attending the STD clinic including Female sex workers, males having sex with males and transgenders harbors the high risk of encountering the sexually transmitted infectionsmaking them more vulnerable in acquiring sexually transmitted infections and also spreading it to the community. AIMS AND OBJECTIVES: 1. To determine the prevalence of sexually transmitted viral infections like Human immunodeficiency virus, Herpes simplex virus 2, Human papilloma virus 16 and 18, hepatitis B and C virus and Gonorrhea, Bacterial Vaginosis, syphilis, vaginal candidiasis and Trichomoniasis among asymptomatic high risk groups of males who have sex with males, Transgenders and female sex workers attending STD clinic in a tertiary care hospital. 2. Comparison of seroprevalence of herpes simplex virus 2 and genital viral shedding among the high risk groups. 3. To determine the prevalence of high risk Human Papilloma virus 16 & 18 viral shedding among the high risk groups. MATERIALS AND METHODS: Specimen collection and processing: Samples from Female sex workers, Males having sex with males, transgender including Endocervical swabs, High vaginal swab, rectal swabs, Urethral swabs, pharyngeal swab and bloodare collected and processed by standard microbiological methods. Pharyngeal and rectal specimens were obtained using cotton swabs. Rectal specimens were obtained either by blind anal swabbing or, via anoscopy.Cervical specimens taken with Dacron swabs are preserved with 70% ethanol solution for molecular analysis. Serological tests done for ELISA HIV, HSV2 IgM and IgG ELISA. Serological testing done for Syphilis with RPR test and Confirmation with Treponema pallidum hemagglutination test (TPHA).Rapid card test done for hepatitis B and Hepatitis C. PCR done from the anogenital swabs for HSV2, HPV 16 and 18. RESULTS: Among the high risk groups taken for study the female sex workers (57%) were the commonest and most of them are unmarried 106(53%) with predominant mode of sexual contact was heterosexual mode (57%) and 41% had unprotected sexual act (without condoms). Out of 200 asymptomatic cases Neisseria gonorrhoeae (1), Trichomonas vaginalis (1), Candida albicans 9 and non albicans 9, Gardernerella vaginalis (4) were isolated. HIV reactivity (8), HbsAg (1) and RPR reactivity (7). IgM, IgG antibodies for HSV 2 is (16) and IgG (67) respectively and PCR for HSV2 is 20. Speciation of the candida species showed candida Human Papilloma Virus 18 E7 and 18 E7 in 4 with one positive for both HPV 16 and 18 co infection. CONCLUSION: Out of 20 PCR positives the IgM was positive in only 16. There was genital viral shedding even in the absence of IgM antibodies. So molecular methods are necessary to know about the genital viral shedding. Continuous efforts are needed to encourage individuals to adopt safer sexual. Prophylactic strategies regarding antiviral therapy for HSV2 infected individuals and vaccination for HPV to be considered in the high risk groups.

Item Type: Thesis (Masters)
Uncontrolled Keywords: Sexually transmitted infections ; High risk groups ; Genital Herpes.
Subjects: MEDICAL > Microbiology
Depositing User: Punitha K
Date Deposited: 23 May 2018 02:59
Last Modified: 27 May 2018 05:23

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