Characterisation of Cell Population Data and Angiogenesis in Myelodysplastic Syndrome

Vishnu Chandra Kumar, A (2015) Characterisation of Cell Population Data and Angiogenesis in Myelodysplastic Syndrome. Masters thesis, Christian Medical College, Vellore.


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AIM OF THE STUDY: 1. To study the cellular alterations in cases of MDS. 2. To study the stromal vascular changes in cases of MDS. OBJECTIVES: 1. To evaluate the role of cell population data (CPD) values obtained through the automated haematology cell counter (Beckman Coulter DxH800) in the diagnosis of MDS. 2. To look for differences in the CPD values among the MDS cases, non MDS cases and healthy controls. 3. To enumerate the Microvessel Density (MVD) in the bone marrow trephine biopsy of patients with MDS, using anti CD34 antibody. 4. To compare the values of MVD between MDS cases and controls. METHODS: The Cell Population Data (CPD) values obtained from the automated cell counter (Beckman Coulter DxH800) of 68 cases with MDS were compared with 155 non MDS patients and 98 normal healthy blood donors. The microvessel density in trephine biopsies of 101 MDS cases was counted using immunohistochemistry (antiCD34 antibody) by “hotspot” method and was compared with 35 normal controls. RESULTS: There was a significant increase in the mean neutrophil volume and mean monocyte volume in MDS cases compared to non MDS and healthy donors (p-value: 0.000 and 0.000 respectively). A significant decrease in the neutrophil scatter and eosinophil scatter was noted in cases of MDS in comparison to non MDS and normal controls (p-value=0.000 and 0.000) respectively). A significant increase in the bone marrow mean MVD in cases of MDS was noted compared to the control marrows (p-value=0.000). CONCLUSION: There is a measurable difference in the cell population data (CPD) in cases of MDS which can be detected at an early stage even before a bone marrow examination is carried out. The increased MVD in MDS is a potential target for antiangiogenic therapy.

Item Type: Thesis (Masters)
Uncontrolled Keywords: Myelodysplastic syndrome (MDS) ; Cell population data (CPD) ; Automated Beckman Coulter DxH800 haematology analyser ; Microvessel density (MVD).
Subjects: MEDICAL > Pathology
Depositing User: Punitha K
Date Deposited: 19 May 2018 15:16
Last Modified: 22 May 2018 18:38

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