Histopathological Study of Myelofibrosis (PMF) and Essential Thrombocythaemia (ET) and Correlation with Clinical and Laboratory features

Pradeep, H (2015) Histopathological Study of Myelofibrosis (PMF) and Essential Thrombocythaemia (ET) and Correlation with Clinical and Laboratory features. Masters thesis, Christian Medical College, Vellore.


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AIM OF THE STUDY: 1. To describe the bone marrow morphology in primary myelofibrosis and essential thrombocythaemia. 2. To develop a scoring system for the diagnosis of these conditions based the various histological parameters seen in bone marrow trephine biopsies in these groups of patients. 3. To determine whether such a scoring system reliably distinguishes between ET and prefibrotic phase of PMF. 4. To compare the blood and bone marrow findings in patients with and without JAK2V617 mutations. METHODS: This was a study done on retrieved bone marrow trephine biopsies in Department of General Pathology, Christian Medical College, Vellore. 134 cases were included in the study, which included 27 cases of ET, 107 cases of PMF, including 13 cases of prefibrotic phase of PMF and correlated with relevant laboratory and clinical parameters. Parameters evaluated: The histomorphological features of all cases was studied in detail with a score applied for each histomorphological parameter with a maximum score of 21. Relevant statistical analysis for Categorical variables and continuous variables were done using chi square test and fisher t test. RESULTS: All cases of ET had a total score of < 10 and scores of prefibrotic MF ranged from 12-17 in our 21 point scoring system thus allowing a distinction between them. JAK2 positivity did have a influence on certain laboratory and histomorphological parameters such JAK2V617 positive cases of PMF had a higher haemoglobin and total leukocyte count at presentation. On histopathology hyperchromatic dysplastic nuclei and small clusters were found more commonly in JAK2V617 positive MF. JAK2WT ET had a higher platelet count at diagnosis and also large clusters being more frequently present in JAK2V617 positive ET.

Item Type: Thesis (Masters)
Uncontrolled Keywords: Myelofibrosis (PMF) ; Essential Thrombocythaemia (ET) ; Clinical and Laboratory features ; Histopathological study.
Subjects: MEDICAL > Pathology
Depositing User: Punitha K
Date Deposited: 19 May 2018 15:16
Last Modified: 22 May 2018 18:26
URI: http://repository-tnmgrmu.ac.in/id/eprint/8009

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