Role of Micronuclei as a Diagnostic Tool in Exfoliative Cytology of Oral Preneoplastic and Neoplastic Conditions among Tobacco Chewers

Suganya, R (2015) Role of Micronuclei as a Diagnostic Tool in Exfoliative Cytology of Oral Preneoplastic and Neoplastic Conditions among Tobacco Chewers. Masters thesis, Coimbatore Medical College, Coimbatore.

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Abstract

BACKGROUND: The assessment of Micronuclei frequency in exfoliated oral epithelial cells have been shown to correlate with severity of the genotoxic damage and seem to increase steadily in order from tobacco chewers with apparently normal mucosa to premalignant and malignant lesions. AIM OF THE STUDY: To evaluate the frequency of micronuclei (MN) in oral exfoliated cells of tobacco chewers with oral premalignant and malignant lesions and comparing them with that of healthy tobacco chewers and controls of non tobacco chewers. Materials and Methods: The study subjects are divided into four groups consisting of tobacco chewers with apparently healthy oral mucosa, premalignant lesions and malignancy, and normal controls, each of 20 cases. The cytosmears are stained with Pap, Giemsa and Crystal violet stains. The micronuclei was identified using Tolbert’s criteria. RESULTS: The frequency of micronuclei is found to be higher in malignant lesions as compared with premalignant lesions and healthy tobacco chewers, and controls. CONCLUSIONS: Hence, micronuclei can be used as a biomarker of genotoxic damage, which is an useful diagnostic as well as prognostic indicator.

Item Type: Thesis (Masters)
Uncontrolled Keywords: Micronuclei ; oral exfoliative cytology ; tobacco chewers ; squamous cell carcinoma ; premalignant lesions ; Papanicolaou ; Giemsa ; Crystal violet stains.
Subjects: MEDICAL > Pathology
Depositing User: Punitha K
Date Deposited: 19 May 2018 15:15
Last Modified: 23 May 2018 01:22
URI: http://repository-tnmgrmu.ac.in/id/eprint/7996

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